Determination of famotidine in human plasma and urine by high-performance liquid chromatography
AbstractAn improved, rapid and specific high-performance liquid chromatographic assay was developed for the determination of famotidine in human plasma and urine. Plasma samples were alkalinized and the analyte and internal standard (cimetidine) extracted with water-saturated ethyl acetate. The extracts were reconstituted in mobile phase, and injected onto a C18 reversed-phase column; UV detection was set at 267 nm. Urine samples were diluted with nine volumes of a mobile phase-internal standard mixture prior to injection. The lower limits of quantification in plasma and urine were 75 ng/ml and 1.0 mg/ ml, respectively; intra- and inter-day coefficients of variation were #10.5%. This method is currently being used to support renal function studies assessing the use of intravenously administered famotidine to characterize cationic tubular secretion in man.
CitationDowling, T. C., & Frye, R. F. (1999). Determination of famotidine in human plasma and urine by high-performance liquid chromatography. Journal of Chromatography B: Biomedical Sciences and Applications, 732(1), 239-243, DOI: 10.1016/S0378-4347(99)00269-8
Identifier to cite or link to this itemhttp://hdl.handle.net/10713/723