Simultaneous determination of p-aminohippuric acid, acetyl-paminohippuric acid and iothalamate in human plasma and urine by high-performance liquid chromatography
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AbstractA sensitive and specific high-performance liquid chromatographic assay was developed for the simultaneous determination of p-aminohippuric acid (PAH), acetyl-p-aminohippuric acid (aPAH), and iothalamate in human plasma and urine. Plasma samples were prepared by protein precipitation with acetonitrile followed by evaporation, reconstitution in mobile phase, and injection onto a C reversed-phase column. Urine samples were diluted with 3 volumes of mobile phase prior to injection. 18 Column effluent was monitored by UV detection at 254 nm. The lower limits of quantification in plasma were 0.5 mg/ l for PAH and aPAH, and 1.0 mg/ l for iothalamate. The within-day and between-day coefficients of variation in plasma and urine were #7.8% for all analytes. This method is well suited for renal function studies using iothalamate and PAH, whether administered as a bolus dose or by continuous infusion, to measure glomerular filtration rate and effective renal plasma flow,respectively.
CitationDowling, T. C., Frye, R. F., & Zemaitis, M. A. (1998). Simultaneous determination of p-aminohippuric acid, acetyl-paminohippuric acid and iothalamate in human plasma and urine by high-performance liquid chromatography. Journal of Chromatography B: Biomedical Sciences and Applications, 716(1-2), 305-313, DOI: 10.1016/S0378-4347(98)00294-1.
Identifier to cite or link to this itemhttp://hdl.handle.net/10713/722