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    PCR Protocol for PfCRT Pyrosequencing

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    Name:
    pfcrt-PCR-for-Pyroseq.pdf
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    Date
    2012-02-09
    Publisher
    University of Maryland School of Medicine. Center for Vaccine Development. Malaria Group
    Type
    Other
    
    Metadata
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    Other Titles
    SUBTITLE: (codons 72, 74, 75, 76, 220,271,326, and 371)
    Description
    "Provides a method for performing PCR on genomic DNA from dried blood spots on Whatman 3MM filter paper ... for subsequent genotyping of pfcrt codons ..."
    Includes the following sections: 1.0 Purpose; 2.0 References; 3.0 Materials; 4.0 Equipment; 5.0 Safety and Precautions; 6.0 Procedure; 6.1 DNA extraction from blood spotted filter papers; 6.2 Setting up PCR Reactions
    Keyword
    pfcrt codon 72
    pfcrt codon 74
    pfcrt codon 75
    pfcrt codon 76
    pfcrt codon 220
    pfcrt codon 271
    pfcrt codon 326
    pfcrt codon 356
    pfcrt codon 371
    pyrosequencing
    University of Maryland, Baltimore. Center for Vaccine Development (CVD)
    Genotyping Techniques--methods
    Plasmodium falciparum--genetics
    Polymerase Chain Reaction
    Sequence Analysis--methods
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    Identifier to cite or link to this item
    http://hdl.handle.net/10713/7042
    Collections
    Center for Vaccine Development and Global Health

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