Recent Submissions

  • Differences in Neuromechanical Factors Affecting Explosive Torque Production During Knee Extension: A Preliminary Comparative Study of Males and Females.

    Lanza, Marcel B.; Frakes, Nathan; Lateef, Shabnam; Baghi, Raziyeh; Rao, Sanjana; Zhang, Li-Qun; Gray, Vicki L. (2024-06-26)
  • Detyrosinated microtubule arrays drive myofibrillar malformations in mdx muscle fibers

    Harriot, Anicca; Altair-Morris, Tessa; Vanegas, Camilo; Kallenbach, Jacob G.; Pinto, Kaylie; Joca, Humberto C.; Moutin, Marie-Jo; Shi, Guoli; Ursitti, Jeanine Anne; Grosberg, Anna; et al. (2024-06-24)
    Altered myofibrillar structure is a consequence of dystrophic pathology that impairs skeletal muscle contractile function and increases susceptibility to contraction injury1–3. In murine Duchenne muscular dystrophy (mdx), myofibrillar alterations are abundant in advanced pathology (>4 months) 1–3, an age where we formerly established densified microtubule (MT) arrays enriched in detyrosinated (deTyr) tubulin as negative disease modifiers impacting cell mechanics and mechanotransduction4,5. Given the essential role of deTyr-enriched MT’s in myofibrillar growth, maintenance, and repair, we examined the increased abundance of these arrays as a potential mechanism for these myofibrillar alterations. Here (see6 ) we find an increase in deTyr-tubulin as an early event in dystrophic pathology (4 weeks) with no evidence myofibrillar alterations. At 16 weeks, we show deTyr-enriched MT arrays significantly densified and co-localized to areas of myofibrillar malformation. Profiling the enzyme complexes responsible for deTyr-tubulin, we identify vasohibin 2 (VASH2) and small vasohibin binding protein (SVBP) significantly elevated in the mdx muscle at 4 wks. Using the genetic increase in VASH2/SVBP expression in 4 wk wild-type mice we find densified deTyrenriched MT arrays that co-segregate with myofibrillar malformations similar to those in the 16 wk mdx. Given that no changes were identified in fibers expressing sfGFP as a control, we conclude that disease-dependent densification of deTyr-enriched MT arrays underscores the altered myofibrillar structure in dystrophic skeletal muscle fibers.
  • Phase 1 Study of Intranasal Fusion Inhibitor RQ-01 for the Treatment of COVID-19

    Kottilil, Shyamasundaran; Kenaston, Kristin; DaSilva-Jardine, Paul; Mitchnick, Mark; Cameron, Kaitlin; Smith, Larry; Recchio, S.; Herce, Henry; Moss, Ronald; Rojas, Luis; et al. (2024-06-13)
  • Comparison of Two Different Blood Incubation and Monitoring Systems

    Smith, Richard Daniel; Paszkiewicz, Gwen; French, Indira; Johnson, J. Kristie (2024-06-10)
    Background: Management of bloodstream infections requires fast and accurate diagnostic testing to better patient outcomes. Blood culture incubation and monitoring systems are critical for identifying positive blood cultures in a timely matter. Reducing incubation time can reduce time to results. Methods: This study compared two blood culture incubation systems and their media, BD BACTEC™ Plus Aerobic/F media (Becton Dickinson) and BacT/Alert®3D FA plus (BioMérieux) to determine their time to detection and recovery rate. In triplicate, 50 matched bacterial strains were inoculated at 10-100 CFU per 0.1ml and incubated on each instrument. Time to positivity was recorded and difference in time to positivity between matched strains was calculated. Wilcoxon signed- rank test was used to calculate significance. Results: All blood cultures were positive within 24 hours. The median time to positivity for all strains was 12 hours and 30 minutes (Interquartile range (IQR): 11:08, 15:55) for BacT/Alert®3D and 11 hours and 13 minutes for BACTEC™ FX (IQR: 10:05, 14:52) (p <0.001) with average difference in time to positivity being 54.6 minutes. For Gram-positive bacteria, median time to positivity was 13 hours and 54 minutes (IQR: 11:44, 16:18) for BacT/Alert®3D and 12 hours and 29 minutes for the BACTEC™ FX (IQR: 10:53, 16:05) (p = 0.02) with an average difference in time to positivity of 57.3 minutes. For Gram- negative bacteria, median time to positivity was 11 hours and 20 minutes (IQR: 10:52, 12:01) for BacT/Alert®3D and 10 hours and 23 minutes for BACTEC™ FX (IQR: 9:37, 11:14) (p < 0.001). Average difference in time to positivity was 61.1 minutes. For fastidious organisms, the median time was 18 hours and 46 minutes for BacT/Alert®3D and 13 hours and 44 minutes for BACTEC™ FX. Conclusions: BACTEC™ FX significantly reduced time to positivity by approximately one hour when compared to BacT/Alert®3D. This reduction in time could improve patient outcomes
  • Development and Validation of a Multiplex Serology Assay for HPV Type-Specific Antibody Detection

    Aslanabadi, Arash; Karimi, Maryam; Powell, Laura; Shutt, Ashley; Crowell, Trevor A.; Bentzen, Søren M.; Cullen, Kevin J.; Kemp, Troy; Pinto, Ligia A.; Schumaker, Lisa; et al. (2024-06-07)
    Human papillomavirus (HPV) is a common viral infection with over 200 identified types, some of which are classified as oncogenic due to their association with various cancers. Among these, HPV16 is notably the most prevalent and is implicated in the majority of cervical cancers, as well as significant proportions of oropharyngeal, anal, penile, vulvar, and vaginal cancers. Prophylactic vaccines, such as the bivalent, quadrivalent, and 9- valent HPV vaccines, are highly effective in preventing infections with the most common oncogenic HPV types. Despite this, vaccine uptake varies globally due to factors such as vaccine availability, cost, cultural acceptance, and lack of awareness. Consequently, many individuals remain unvaccinated and at risk for HPV infection and its associated cancers. Catch-up vaccination programs are essential for reaching those who missed routine vaccination during adolescence. However, identifying individuals who are seronegative for HPV-16 and other oncogenic types is crucial for the efficient allocation of vaccines, especially in resource-limited settings. Current serological assays are often limited in scope and require large sample volumes, making them less practical for widespread use. We aimed to develop a multiplex serology assay that can simultaneously detect antibodies against multiple HPV types from a small sample volume, providing a valuable tool for targeted vaccination strategies. Also, our objective was to test the performance of our HPV type-specific serology assay using an Internationally defined proficiency panel developed at the National Cancer Institute (NCI) Frederick Laboratory
  • The relationship between alcohol consumption and gut microbiome diversity in individuals with AUD and controls

    Doty, Carolyn; Grant-Beurmann, Silvia; Harrington, Valeria; Kelly, Deanna L.; Bennett, Melanie; Leggio, Lorenzo; Brandler, Brian; Brady, Daniel; Rincon, Natalia; Roche, Daniel (2024-06-06)
  • ALL JOKES ASIDE, A CASE FOR CLOZAPINE MICRODOSING

    Richardson, Elizabeth; Reeves, Gloria; McDonald, Kathryn; Ehret, Megan J.; Kelly, Deanna L. (2024-06-06)
  • Early intervention of psychosis for immigrant patients at a psychosis clinic versus an immigrant-centered community clinic

    Schin, Christina; McDonald, Kathryn; Fitzgerald, John; Akbar, Muhammad; Malick, Rida; Hackman, Ann; Okuzawa, Nana; Reeves, Gloria (2024-06-06)
  • Novel regulation of cell surface membrane proteins through selective autophagy

    Sidibe, Djeneba; Singh, Manisha; Bustos Segura, America J.; Harraz, Maged M. (2024-06-06)
  • Upregulation of hippocampal aromatase in the aged male mouse brain

    Postle, Abagail F.; Georgiou, Polymnia; Clark, Sarah M., Ph.D.; Gould, Todd D. (2024-06-06)
  • Ablation of the Hv1 proton channel in microglia confers neuroprotection after spinal cord injury in male mice

    Wang, Zihui; Lei, Zhuofan; Krishnamachary, Balaji; Li, Yun; Li, Hui; Wu, Long-Jun; Wu, Junfang (2024-06-09)
    The voltage-gated proton channel Hv1 is a recently cloned ion channel that rapidly removes protons from depolarized cytoplasm and is highly expressed in the immune system. In the CNS, Hv1 is expressed by resting microglia but not neurons or astrocytes in the mouse brain. Microglial Hv1 regulates intracellular pH and aids in NOX2-dependent generation of reactive oxygen species (ROS). Thus, Hv1 is a unique target for controlling multiple NOX activities and ROS production. Recently, we observed rapid and persistent upregulation of Hv1 in the injured spinal cord after a moderate contusion; the remained up to 8 months post-injury1,2. Our revelation studies point to an acute neuroprotection phenotype when Hv1 is genetically deleted including infiltrating immune cells, indicating a critical role of Hv1 signaling in SCI pathophysiology1. However, neither the precise cellular mechanisms underlying this finding nor critical role of Hv1 in the pathophysiology of SCI are fully understood. We hypothesize that microglial Hv1 functions as a key mechanism in neuroinflammation, thus affecting long-term neurological outcome after SCI.
  • Novel Regulation of the Dopamine Transporter by Cocaine-Induced Autophagy

    Bustos Segura, America J.; Shishikura, Maria; Harraz, Maged M. (2024-06-06)
  • Inflammatory pDCs impair stem like CD8 T cells during chronic HIV 1 infection

    Li, Guangming; Lou, Yaoxian; Ma, Jianping; Yu, Haisheng; Tsahouridis, Ourania; Mathur, Poonam; Su, Lishan (2024-06-08)
  • Fibroblast reticular cells crosstalk with regulatory T cells through the regulation of laminin α4 and α5 in the lymph node

    Kong, Dejun; Shirkey, Marina W.; Lee, Young; Kensiski, Allison; Piao, Wenji; Wu, Long; Luo, Shunqun; Pettit, Sarah; Zapas, Greg; Abdi, Reza; et al. (2024-06-01)
  • Immunologic Scarring Caused by Stimulation with Unrelated Alloantigen or Inflammation Impairs Cardiac Allograft Survival

    Kensiski, Allison; Zhang, Timothy; Kish, Danielle; Gavzy, Samuel J.; Shirkey, Marina W.; Fairchild, Robert Leslie, 1953-; Bromberg, Jonathan Scott; Wu, Long (2024-06-02)
  • MMP-9 enhanced Mesenchymal stem cells for the treatment of liver fibrosis

    Verma, Anjali; Kamberi, Shani; Aseer, Kanikkai Raja; Juan, Daniel; Mas, Valeria; Meier, Raphael P.H. (2024-06-01)
  • Identification of Trauma-Exposed Endophenotypes in Individuals with Schizophrenia Related Disorders and its Relevance to Plasma Neuroactive Steroids

    Milrad, Sara; Pinna, Graziano; Glassman, Matthew; Roche, Daniel; Davis, John; Vyas, Gopal; Kelly, Deanna L. (2024-06-06)

View more