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dc.contributor.authorNnabue, Thaddeus
dc.date.accessioned2016-06-28T18:18:12Z
dc.date.available2016-06-28T18:18:12Z
dc.date.issued2016
dc.identifier.urihttp://hdl.handle.net/10713/5476
dc.descriptionUniversity of Maryland, Baltimore. Medical and Research Technology. M.S. 2016en_US
dc.description.abstractPACAP is a pleiotropic polypeptide with cytotrophic, anti-inflammatory, and anti-apoptotic activities. Mitoxantrone (MTX) is a chemotherapeutic drug used for treatment of multiple sclerosis. My hypothesis is that PACAP will protect the granulosa cells of the ovarian follicle from cytotoxic cell death caused by MTX treatment. A total number of 27 mice were assigned to 3 groups; MTX, MTX + PACAP, and control. TUNEL staining, caspase-3 assay, and H&E staining were used to visualize the granulosa cells in ovary sections. H&E staining showed that MTX treatment reduced the number of developing follicles. Positive TUNEL in granulosa cells of ovary treated with MTX confirmed cell damage by MTX. Follicles in the MTX + PACAP group were TUNEL negative. The caspase-3 staining was negative in all groups. These observations suggest that MTX causes granulosa cell death from DNA damage but not due to apoptosis, and PACAP protects the granulosa cells from DNA damage.en_US
dc.language.isoen_USen_US
dc.subjectchemotherapy-induced amenorrheaen_US
dc.subjectPACAPen_US
dc.subject.meshApoptosisen_US
dc.subject.meshGranulosa Cellsen_US
dc.subject.meshMitoxantroneen_US
dc.subject.meshMultiple Sclerosisen_US
dc.subject.meshPituitary Adenylate Cyclase-Activating Polypeptideen_US
dc.titlePituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Protects the Ovary of Mice Against Mitoxantrone Cytotoxicityen_US
dc.typedissertationen_US
dc.contributor.advisorMerchenthaler, Istvan
dc.contributor.advisorVucenik, Ivana
refterms.dateFOA2019-02-20T18:21:06Z


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