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    Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Protects the Ovary of Mice Against Mitoxantrone Cytotoxicity

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    Author
    Nnabue, Thaddeus
    Advisor
    Merchenthaler, Istvan
    Vucenik, Ivana
    Date
    2016
    Type
    dissertation
    
    Metadata
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    Abstract
    PACAP is a pleiotropic polypeptide with cytotrophic, anti-inflammatory, and anti-apoptotic activities. Mitoxantrone (MTX) is a chemotherapeutic drug used for treatment of multiple sclerosis. My hypothesis is that PACAP will protect the granulosa cells of the ovarian follicle from cytotoxic cell death caused by MTX treatment. A total number of 27 mice were assigned to 3 groups; MTX, MTX + PACAP, and control. TUNEL staining, caspase-3 assay, and H&E staining were used to visualize the granulosa cells in ovary sections. H&E staining showed that MTX treatment reduced the number of developing follicles. Positive TUNEL in granulosa cells of ovary treated with MTX confirmed cell damage by MTX. Follicles in the MTX + PACAP group were TUNEL negative. The caspase-3 staining was negative in all groups. These observations suggest that MTX causes granulosa cell death from DNA damage but not due to apoptosis, and PACAP protects the granulosa cells from DNA damage.
    Description
    University of Maryland, Baltimore. Medical and Research Technology. M.S. 2016
    Keyword
    chemotherapy-induced amenorrhea
    PACAP
    Apoptosis
    Granulosa Cells
    Mitoxantrone
    Multiple Sclerosis
    Pituitary Adenylate Cyclase-Activating Polypeptide
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/5476
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    Theses and Dissertations School of Medicine
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