• Login
    View Item 
    •   UMB Digital Archive
    • School, Graduate
    • Theses and Dissertations All Schools
    • View Item
    •   UMB Digital Archive
    • School, Graduate
    • Theses and Dissertations All Schools
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Acetylation of α-tubulin and its Role in Aggressive and Metastatic Breast Cancer

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Boggs_umaryland_0373D_10558.pdf
    Size:
    3.261Mb
    Format:
    PDF
    Download
    Author
    Boggs, Amanda
    Advisor
    Martin, Stuart S.
    Date
    2014
    Type
    dissertation
    
    Metadata
    Show full item record
    Other Titles
    Acetylation of Alpha-tubulin and its Role in Aggressive and Metastatic Breast Cancer
    Abstract
    Metastatic breast cancer presents a therapeutic challenge, since existing treatments largely target primary tumor growth instead of metastatic spread. Because metastasis is the leading cause of breast cancer-related death, we must investigate new targets for the treatment of disseminated disease. This study describes a novel role for α-tubulin acetylation in metastatic breast cancer. We report that metastatic breast cancer cells have high α-tubulin acetylation that is maintained under suspended conditions and extends along microtentacles, tubulin-based protrusions that promote cell-cell and cell-substrate reattachment. Mutation of the acetylation site on α-tubulin and enzymatic modulation of this post-translational modification has a significant impact on microtentacle frequency and suspended tumor cell reattachment. Reducing α-tubulin acetylation in metastatic breast tumor cell lines also significantly inhibits migration, but does not affect proliferation. Investigating the translational importance of this modification in over 140 breast cancer patients' matched primary and metastatic tumors, we find that α-tubulin acetylation is maintained and in many cases increased in nodal metastases, relative to the primary tumor. We also discover a strong correlation between α-tubulin acetylation and the aggressive basal-like breast cancer subtype in a large cohort of breast cancer patients. These data suggest α-tubulin acetylation may promote a more metastatic phenotype through its effects on reattachment and migration while serving as a marker of an aggressive breast cancer subtype.
    Description
    University of Maryland, Baltimore. Molecular Medicine. Ph.D. 2014
    Keyword
    microtentacles
    Breast--Cancer
    Metastasis
    Acetylation
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/4187
    Collections
    Theses and Dissertations School of Medicine
    Theses and Dissertations All Schools

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.