• Login
    View Item 
    •   UMB Digital Archive
    • School, Graduate
    • Theses and Dissertations All Schools
    • View Item
    •   UMB Digital Archive
    • School, Graduate
    • Theses and Dissertations All Schools
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Acute ROS in Cardiac Calcium Signaling

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Bamgboye_umaryland_0373D_10493.pdf
    Size:
    15.83Mb
    Format:
    PDF
    Download
    Author
    Bamgboye, Moradeke A.
    Advisor
    Lederer, W. Jonathan
    Date
    2014
    Type
    dissertation
    
    Metadata
    Show full item record
    Abstract
    Modulation of cardiac function usually involves changes in cellular Ca2+ signaling. Reactive oxygen species (ROS) have been implicated in effecting changes in cardiac Ca2+ signaling. It has been unclear, however, exactly what these changes are and if they are ultimately detrimental or beneficial. The work reported here investigates the effect of acute application of H2O2 in low concentrations on Ca2+ signaling in the heart cell. The driving hypothesis is that ROS in short bursts is a non - detrimental physiological signal that fine tunes Ca2+ signaling. To this end the effect of ROS is investigated in 3 modules. i. the effect of rapid application of 100 μM H2O2 on Ca2+ signaling in heart cells, ii. the effect of H2O2 on Ca2+ signaling during β-adrenergic activation in heart cells and finally to examine endogenously generated ROS, iii. Ca2+ signaling in a murine model of NOX2 overexpression (NOX2 is a ROS generating enzyme). Using a combination of confocal imaging of fluorescent Ca2+ dyes and electrophysiological techniques such as whole cell voltage clamp and current clamp; Ca2+ sparks, Ca2+ transients, ICa, Sarcoplasmic Reticulum (SR) Ca2+ load and Sarcoplasmic/Endoplasmic Reticulum Ca2+ ATPase (SERCA) function were measured in all three paradigms listed above. The work shows that low concentrations of H2O2 for a brief period deplete SR load without affecting other parameters of Ca2+ signaling, and this depletion of SR load is prevented by β-adrenergic activation. The results further our understanding of ROS modulation of Ca2+ signaling and lays some groundwork for further exploration into the pathways by which ROS may interact with other modifiers of the Ca2+ signaling machinery of the cardiac cell.
    Description
    University of Maryland, Baltimore. Molecular Medicine. Ph.D. 2014
    Keyword
    cardiac
    ROS
    Calcium
    Electrophysiology
    Reactive Oxygen Species
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/4111
    Collections
    Theses and Dissertations School of Medicine
    Theses and Dissertations All Schools

    entitlement

     
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.