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    Breast Tumor Stem Cells Have Increased Microtentacles That Can Be Targeted Therapeutically with Curcumin

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    Author
    Charpentier, Monica S.
    Advisor
    Martin, Stuart S.
    Date
    2014
    Type
    dissertation
    
    Metadata
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    Abstract
    Cancer stem-like cells (CSC) and circulating tumor cells (CTCs) have related properties associated with distant metastasis, but the mechanisms through which CSCs promote metastasis are unclear. In this study, we report that breast cancer cell lines with more stem-like properties display higher levels of microtentacles (McTNs), a type of tubulin-based protrusion of the plasma cell membrane which forms on detached or suspended cells and aid in cell reattachment. We hypothesized that CSCs with large numbers of McTNs would more efficiently attach to distant tissues, promoting metastatic efficiency. The naturally occurring stem-like subpopulation of the HMLE breast cell line presents increased McTNs compared to its isogenic non-stem-like subpopulation, when these subpopulations are separated by flow cytometry. This increase in McTNs was supported by elevated α-tubulin detyrosination and vimentin protein levels and organization. Increased McTNs in stem-like HMLEs promoted a faster initial reattachment of suspended cells that was inhibited by the tubulin-directed drug, colchicine, confirming a functional role for McTNs in stem cell reattachment. Moreover, live cell confocal microscopy showed that McTNs persist in breast stem cell mammospheres as flexible, motile protrusions on the surface of the mammosphere. While exposed to the environment, they also function as extensions between adjacent cells along cell-cell junctions. We found that treatment with the breast CSC-targeting compound curcumin rapidly extinguished McTNs in breast CSCs, preventing reattachment from suspension. Together, our results support a model in which breast CSCs with cytoskeletal alterations that promote McTNs can mediate attachment and metastasis but might be targeted by curcumin as an anti-metastatic strategy.
    Description
    University of Maryland, Baltimore. Molecular Medicine. Ph.D. 2014
    Keyword
    cancer stem cells
    Cytoskeleton
    Detyrosinated Tubulin
    microtentacles
    Metastasis
    Curcumin
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/4081
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