• In vitro regulation of Human Breast Cancer 1 (BRCA 1) gene expression by Bisphenol A

      Jones, Nicole Danielle; Jones, Laundette P. (2012)
      Abstract Title of Thesis: In vitro regulation of Human Breast Cancer 1 (BRCA1) gene expression by Bisphenol A Nicole Jones, Master of Science, 2012 Thesis Directed by: Laundette Jones, PhD Assistant Professor Department of Pharmalogy School of Medicine University of Maryland Breast Cancer is the second leading cause of death among women. Risk factors for breast cancer include lifetime exposure to estrogen, hereditary genetic mutations and environmental influences. According to researchers, Bisphenol A (BPA), an environmental chemical used in polycarbonate plastics, may be linked to the development of breast cancer. However, it is unclear how this chemical plays a role in breast cancer development. Recent microarray studies from Singleton et al., demonstrated that BRCA1 gene expression is decreased by BPA in MCF7 cells over expressing estrogen receptor. It is known that decreased expression of BRCA1 accelerates growth of mammary epithelial cells and is often decreased in sporadic breast cancer progression. We hypothesize BPA, like estrogen, could alter BRCA1 expression. Our data suggest that treating cells with BPA at levels comparable to human exposures alters BRCA1 expression and the changes in BRCA1 expression by BPA is concentration dependent. This study highlights the importance of conducting research to understand how BPA alteration of BRCA1 impacts its normal cellular function.