Now showing items 1-20 of 673

    • Innovative Approach to Graduate Capstone Project Development

      Mishler, Oksana; Bress, Lisa; Burgee, Suzanne (2024-06-01)
    • Building a database of membrane glycolipid barcodes using a rapid purification-free and target-agnostic MALDI-TOF MS lipid analysis technique

      O'Keefe, Ian; Vu, Linda; Overly, Moira; Yang, Hyojik; Smith, Richard Daniel; Sumner, Kylie P.; Sherman, Matthew; Goodlett, David Robinson, 1960-; Sweet, Charles R.; Ernst, Robert K. (2024-06-02)
    • A multimodal system for lipid A structural analysis from a single colony

      Yang, Hyojik; O'Keefe, Ian; Smith, Richard Daniel; Sumner, Kylie P.; Sherman, Matthew; Goodlett, David Robinson, 1960-; Sweet, Charles R.; Ernst, Robert K. (2024-06-02)
      ipid A, the hydrophobic anchor of lipopolysaccharide (LPS) is a potent sepsis-inducing molecule that stimulates human immune responses. The structural diversity of lipid A is correlated both with pathogenic capability and adaptation to the unique bacterial growth environments. Thus, understanding the structure of lipid A is important in fundamental microbiology and host-pathogen interactions. Previously, our group developed Fast Lipid Analysis Technique (FLAT) which employs an on-surface extraction combined with conventional Matrix-Assisted Laser Desorption/Ionization Mass Spectrometer (MALDI-MS), allowing for rapid structural characterization of lipid A directly from raw biomass of bacterial cells. Here, we employ tandem MS in both polarities, negative-ion (FLATn) and positive-ion (FLATn+) modes, to elucidate the structure of lipid A of various Gram-negative bacteria form single colonies.

      Schneider, Monica, D.D.S., M.S.; Kulo, Violet (2024-04-12)
      The purpose of this qualitative phenomenological study is to understand predoctoral dental students’ lived experiences with service-learning (SL) in general dentistry and explore how these experiences influence their perceptions of community service and future professional practice. Purposive sampling will be used to identify predoctoral students from dental schools with SL rotations that satisfy the study criteria. A SL rotation in this study is defined as an experiential learning approach that aims to encourage civic awareness and participation. The sample for this study is senior predoctoral dental students enrolled in an accredited dental school program in the United States that participate in a SL clinical rotation during their dental education training. Data will be collected by performing virtual semi-structured interviews. Data analysis includes an iterative process and inductive approach where a thematic framework is developed to create an initial list of codes and analyze data to develop themes and descriptions of the participants’ experiences and report the essence of the phenomenon. SL experiences in dental schools vary in their organization, structure, type of assessments used, length of the experience, and type of learning site. It is not clear whether students get comparable SL experiences that might impact their perspectives on community service and future professional practice. In addition, this study will expand the understanding of Mezirow’s (2000) transformative learning theory and add to the base of the theoretical literature. Particularly, dental students SL clinical experiences might present a disorienting dilemma which is connected to the initiation of the transformative learning process.
    • Role of Gut Microbiota in Osteoclast Regulation and Skeletal Homeostasis

      Tanwar, Himanshi; Gnanasekaran, Jeba Mercy; Allison, Devon Lea; Thumbigere-Math, Vivek (2024-01-16)
    • Factors affecting tooth loss among diabetic patients at an academic institution

      Zimmerman, Erica; Oh, Se-Lim; Chen, Hegang (2024-03-06)
    • Influence of Imidazolium-Based Ionic Liquids on Acrylic Denture Base Materials

      Garcia, Isadora Martini; Virgina, Serra de Souza; Scholten, Jackson Damiani; Sampaio de Melo, Mary Anne; Mezzomo Collares, Fabrício (2024-03-13)
    • Novel Bioactive Low-Shrinkage-Stress Composite with Antibacterial and Remineralization Properties

      ALHUSSEIN, ABDULLAH; Xu, Huakun H. (2023)
      Methacrylate-based resin composites are frequently employed in dentistry for their aesthetic qualities, durability, and adhesive properties. Nevertheless, these restorations generally exhibit a lifespan of 5 to 10 years, with recurrent caries and tooth fractures being primary failure factors. Marginal integrity and the absence of bioactivity at the tooth-restoration junction contribute to recurrent caries development. Consequently, this dissertation endeavors to introduce a novel bioactive low-shrinkage-stress nanocomposite, featuring dimethylaminododecyl methacrylate (DMADDM) as an antibacterial agent, as well as remineralization nanoparticles of calcium fluoride (nCaF2) and nanoparticles of amorphous calcium phosphate (NACP), with the potential of increase the longevity of dental restoration and protect tooth structure. All novel formulations of low-shrinkage-stress composite were subjected to a series of mechanical, antibacterial, cytocompatibility, and ion release assessments. First, we investigated the optimum concentration of DMADDM that can be incorporated with a low-shrinkage-stress composite without compromising mechanical properties. We found that incorporation of up to 5% DMADDM into a low-shrinkage stress composite efficiently inhibited Streptococcus mutans (S. mutans) biofilm commonly associated with secondary caries. This potent antibacterial effect is achieved while maintaining excellent mechanical properties and minimizing polymerization shrinkage stress, potentially improving the long-term success of dental restorations. Next, we investigated the antibacterial and cytocompatibility of the incorporation of 3% DMADDM with 20% nCaF2 or 20% NCAP into a low-shrinkage-stress nanocomposite. We found that incorporating DMADDM with either nCaF2 or NACP into a low-shrinkage-stress nanocomposite provides a potent antibacterial effect against S. mutans biofilm while maintaining excellent mechanical properties. In addition, the novel formulations demonstrated excellent biocompatibility against human gingival fibroblasts and dental pulp stem cells. Lastly, we investigated the ions release and antibacterial properties against a salivary biofilm for our innovative formulations. The innovative mixture of DMADDM, NACP, and nCaF2 demonstrated strong antibiofilm effects on salivary biofilm, while concomitantly releasing a significant amount of remineralizing ions. This nanocomposite is a promising dental material with antibiofilm and remineralization capacities, with the potential to reduce polymerization-related microleakage and recurrent caries.
    • The Alma Mater 1974

      Baltimore College of Dental Surgery, Dental School of University of Maryland. Alumni Association. (1974-04)
    • The Alma Mater 1973

      Baltimore College of Dental Surgery, Dental School of University of Maryland. Alumni Association. (1973-04)
    • The Alma Mater 1961

      Baltimore College of Dental Surgery, Dental School of University of Maryland. Alumni Association. (1961-06)
    • The Alma Mater 1959

      Baltimore College of Dental Surgery, Dental School of University of Maryland. Alumni Association. (1959)
    • Effects of Environmental Enrichment on Visceral Sensitivity and Brain Connectivity in a Mouse Model of Chronic Overlapping Pain Conditions

      MIchael, Keaser; Hanson, Shelby; Scott, Alison June; Traub, Richard J.; Da Silva, Joyce T. (2023-11-11)
    • Metformin and other metabolic inhibitors attenuate neuropathic pain and tumor growth in mice with paraneoplastic syndrome and CIPN

      Kuppusamy, Panjamurthy; Haque, Mamunui; Melemedijian, Ohannes (2023-11-15)
      Chemotherapy-induced peripheral neuropathy (CIPN) and paraneoplastic neurological syndrome are two conditions that can cause significant pain and discomfort in cancer patients. CIPN is a common side effect of certain chemotherapeutics and can result in numbness, tingling, and pain. Paraneoplastic neurological syndrome, on the other hand, is a rare disorder that occurs when cancer-fighting antibodies attack parts of the nervous system. Both neuropathies can persist which can adversely affect the quality of life and the rehabilitation of cancer patients. Unfortunately, therapies that can alleviate tumor or chemotherapy-induced neuropathic pain that do not interfere with tumor growth do not currently exist. The main goal of this study was to identify a therapeutic strategy that can achieve both anti-tumor and analgesic effects. The chemotherapeutic, bortezomib, has been shown to induce aerobic glycolysis in sensory neurons which lead to bortezomib-induce neuropathic pain. Aerobic glycolysis is also a hallmark of cancer cells, suggesting a common metabolic vulnerability. Paraneoplastic neuropathies are commonly associated with lung cancers. Hence, we used Lewis Lung Carcinoma cells (LLC1) to develop a mouse model of paraneoplastic neuropathy. We hypothesized that blocking metabolic pathways could alleviate CIPN and paraneoplastic neuropathic pain without compromising on tumor control. To test our hypothesis, we demonstrated that mice implanted with LLC1 developed significant allodynia. Treatment with bortezomib attenuated tumor growth but exacerbated the neuropathic pain. However, co-treatment with metformin, which blocks bortezomib- induced aerobic glycolysis in sensory neurons and prevents CIPN, attenuated both tumor growth and neuropathic pain. Similarly, inhibition of lactate dehydrogenase and pyruvate dehydrogenase kinase with oxamate and dichloroacetate respectively, also reduced tumor growth and pain. These results suggest that targeting metabolic pathways is a promising strategy to improve oncologic outcomes and alleviate neuropathic pain in cancer patients.
    • MDental 2023

      University of Maryland, Baltimore. School of Dentistry, 2023
    • The quality of information on oral hygiene instructions for orthodontic patients in TikTok videos

      Dorfmann, Sabrina; Schneider, Monica, D.D.S., M.S. (2023)
      Objective: The study aimed to assess the reliability, content, quality, and overall usefulness of orthodontic oral hygiene instructions in TikTok videos. Methods: The final videos were evaluated for content, reliability, quality, and usefulness by using a content domain checklist, DISCERN questionnaire, and Global Quality Scale (GQS). Descriptive video characteristics were also recorded. Results: Dental professionals uploaded 70.00% of the TikTok videos, with orthodontists responsible for 39%. The videos had average scores of 2.19 for DISCERN, 3.96 for content, 2.14 for GQS, and 6.10 for usefulness. Video length was significantly associated with content scores (r = 0.3553, P = 0.0003), usefulness (r=0.3553, P = 0.0003), and quality (GQS) (r=0.2620, P=0.0085). Conclusion: Most TikTok videos on orthodontic oral hygiene were uploaded by dental professionals. Reliability, content, and quality of the videos were all poor. Videos were considered slightly useful. Longer videos were significantly correlated with higher content, higher quality, and increased usefulness.
    • Addressing Barriers to Dental Care Access for Undocumented Pregnant Moms

      Young, Mariela; Harasta, Qian; Dedgeba, Hikam (2023-06-09)
    • Perception of lip attractiveness: a survey of orthodontists, general dentists, and laypersons

      McCormack, Katie; Copello, Flavio (2023)
      We aim to investigate the proposed standards of ideal lip proportions and compare those standards to what different people view as most attractive. A 16-question survey was distributed via Qualtrics to orthodontists, general dentists, and laypersons asking demographics as well as opinion-based questions regarding a series of facial photographs. Photographs were digitally altered using Adobe Photoshop to produce different dimensions of the lips from both profile and frontal views. Participants were asked to rate the perceived attractiveness of the lips shown. Regardless of occupation, individuals preferred the appearance of slightly protrusive or thicker lips. Retrusive and thin lip varieties had the lowest median attractiveness scores. Average values for lip projection and thickness were not found to be mutually agreed upon across occupation types (p<0.05) and average lip thickness was not mutually agreed upon between age groups of orthodontists (p=0.004). Based on the results of this study, care should be taken not to produce retrusive or thin lips during orthodontic treatment. Acceptance of a slightly thick or protrusive lip profile may be a more esthetic or well-accepted patient outcome.