• Abutment Material Affects the Attachment of Co-cultured Fibroblasts and Keratinocytes

      Tibbs, Maria; Saito, Hanae (2019)
      Creating a stronger transmucosal seal around the implant abutment may help prevent epithelial downgrowth and resultant crestal bone loss. Most studies focus on titanium and zirconium, but provisional restorations are gaining popularity and these materials require further study. Previous in vitro models utilize a monoculture technique to understand cell behavior, which makes direct intercellular comparisons difficult. Our first aim was to develop a co-culture of human gingival fibroblasts and human oral keratinocytes. Then, cell attachment, proliferation, and migration across six commercially available abutment materials was ascertained and comparisons drawn. Discs made of smooth titanium, (control), rough titanium, CAD/CAM poly (methylmethacrylate), poly ether etherketone, smooth zirconium, and rough zirconium were chosen. Preliminary results indicate that at various time points, significant differences in fibroblast and keratinocyte proliferation and attachment exist among abutment materials.
    • Evaluation of a Poly(Lactic-Co-Glycolic) Acid-Coated β-Tricalcium Phosphate Bone Substitute for Alveolar Ridge Preservation: Multicenter Randomized Control Trial

      West, Brandon M.; Saito, Hanae (2019)
      Purpose: The aim of this study was to determine whether the efficacy of PLGA-β-TCP in Alveolar Ridge Preservation (ARP) with histological analysis of the extraction socket following four months healing was similar to FDBA + CP as the control. Materials and Methods: 45 adult patients in this multicenter randomized controlled clinical trial were randomly assigned into treatment group A [PLGA-β-TCP] or group B [FDBA + CP] for ARP of a single extraction socket. Results: 45 bone core samples were harvested. Vital bone was present in intimate contact with the surface of β-TCP graft particles. Histologic measurements revealed no statistically significant difference between the test and control groups in percent vital bone after four months healing. Conclusion: This investigation demonstrated the similar efficacy of PLGA- β-TCP to FDBA as a bone substitute in ARP.