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dc.contributor.authorJoyner, Katherine Angel
dc.date.accessioned2013-09-09T14:57:04Z
dc.date.available2014-07-09T12:07:58Z
dc.date.issued2013
dc.identifier.urihttp://hdl.handle.net/10713/2978
dc.descriptionUniversity of Maryland, Baltimore. Pharmaceutical Sciences. Ph.D. 2013en_US
dc.description.abstractHydrogels are a unique class of materials lying at the interface of biology and engineering. In the development of this class of materials, understanding the underlying molecular mechanisms that dictate mechanical and structural properties is critical. Non- covalent interactions have been shown here to specifically modulate the properties of peptide hydrogels. A pair of oppositely charged peptides, that when mixed together self-assemble in to a hydrogel network were used in these studies. It was demonstrated using NMR signal decay, that electrostatic interactions are the primary contributions to peptide assembly into a hydrogel network. A decrease of electrostatic interactions results in a decrease in mechanical strength. Also, the ability for hydrogels to form elongated fibrous networks was prevented with the decrease in electrostatic interactions. We further investigated the role of peptide terminal chemistries in hydrogel design. With simple chemical modifications (N-termini acetyl- (COCH3) and amino- (NH2); C-terminal -carboxyl (COOH) and -amide (NH2)) regulation of the mechanical and structural properties was demonstrated. Generally the mechanical strength and fiber structure did not vary, however gelation kinetics and fiber characteristics were directly related to changes in peptide terminal chemistries. These results show the utility of non - covalent interactions in modulating peptide hydrogel structural and mechanical properties in the development of biomedical materials. We additionally examined the chromatographic analysis and separation involved in the development of peptide-based biomaterials through comparison of fluorocarbon and hydrocarbon columns and eluents in the retention and separation of non-fluorinated analytes. The results from these studies showed that fluorocarbon columns displayed the best separation and have a significant potential for use in development.en_US
dc.language.isoen_USen_US
dc.subjectbiomaterialsen_US
dc.subjectdynamic rheologyen_US
dc.subjectHPLCen_US
dc.subjectnon-covalent interactionsen_US
dc.subjectpeptide hydrogelsen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshHydrogelsen_US
dc.subject.meshScattering, Small Angleen_US
dc.subject.meshBiocompatible Materialsen_US
dc.titleNon-covalent Interactions in Peptide Hydrogels: A Mechanism for Mechanical and Structural Controlen_US
dc.typedissertationen_US
dc.contributor.advisorYu, Yihua Bruce
dc.identifier.ispublishedNoen_US
dc.description.urinameFull Texten_US
refterms.dateFOA2019-02-21T03:23:38Z


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