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    The role of STAT6 modulation of natural and inducible Tregs during Allergic Lung Inflammation

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    Author
    Dorsey, Nicolas
    Advisor
    Keegan, Achsah D.
    Date
    2013
    Type
    dissertation
    
    Metadata
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    Abstract
    IL-4 plays a central role in allergic responses by activating the STAT6 pathway. Several studies indicate that regulatory T-cells (Treg) are modulated by IL-4 in vitro. We previously showed that STAT6-/- mice are highly resistant to allergic lung inflammation even when wild type Th2 effectors were provided and that they have increased numbers of Tregs. However, the role of STAT6 in modulating Tregs in vivo during allergic lung inflammation has not been thoroughly investigated. We hypothesized that IL-4-induced activation of STAT6 suppresses natural and inducible Treg differentiation, leading to enhanced allergic lung inflammation. Using a GFP marker for Tregs, we found that STAT6-/- mice have increased frequencies and total numbers of natural (n) Tregs in vivo and also increased frequencies of in vitro generated iTregs compared to STAT6-sufficient mice. Additionally, utilization of Helios and Nrp1 as markers for nTregs revealed a similar result. Taken together, these results suggest that STAT6-/- mice are highly resistant to Th2-driven inflammation because of their elevated numbers of Tregs. To test this hypothesis, STAT6-/-, STAT6xRAG2-/- and RAG2-/- mice were subjected to OVA-sensitization and challenge following adoptive transfer of OVA-specific, wild type Th2 effectors with or without prior Treg depletion/ inactivation using anti-CD25 (PC61). As expected, STAT6-/- mice were highly resistant to airway inflammation and remodeling. In contrast, allergic lung inflammation was partially restored in STAT6-/- mice treated with PC61 to levels observed in STAT6xRAG2-/- mice. In some cases, STAT6xRAG2-/- mice were also given nTregs along with Th2 effectors. Adoptive transfer of nTregs caused a substantial reduction in BAL eosinophil composition and suppressed airway remodeling and T-cell migration into the lung in STAT6xRAG2-/- mice to levels comparable to those in STAT6-/- mice. We also analyzed the contribution of another IL-4-activated signaling mediator, insulin receptor substrate 2 (IRS2). In contrast to STAT6, IRS2 suppressed iTreg expansion, but not nTreg expansion in vivo. These results illustrate that two signaling pathways activated by IL-4, STAT6 and IRS2, differentially antagonize Tregs in vivo. STAT6 and IRS2 both suppress iTregs, while only STAT6 suppresses nTregs. Furthermore, we demonstrate that STAT6 suppresses Tregs in vivo thereby promoting allergic airway inflammation.
    Description
    University of Maryland, Baltimore. Molecular Microbiology and Immunology. Ph.D. 2013
    Keyword
    regulatory T cells
    Allergy
    Asthma
    Hypersensitivity
    Lung
    T-Lymphocytes, Regulatory
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/2973
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    Theses and Dissertations All Schools
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