• Login
    View Item 
    •   UMB Digital Archive
    • School, Graduate
    • Theses and Dissertations All Schools
    • View Item
    •   UMB Digital Archive
    • School, Graduate
    • Theses and Dissertations All Schools
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Mesothelial cell expression of omentin, both a target and a modulator of chronic inflammation

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Erwin_umaryland_0373N_10439.pdf
    Size:
    7.234Mb
    Format:
    PDF
    Download
    Author
    Erwin, Sharis Ashley-Evette
    Advisor
    McLenithan, John C.
    Date
    2013
    Type
    dissertation
    
    Metadata
    Show full item record
    Abstract
    Omentin is a GPI-anchored 38-kDa secreted insulin-sensitizing glycoprotein that is expressed in the mesothelial cells of the stromal vascular fraction (SVF) of visceral adipose tissue. Mesothelial cells expressing omentin are part of the innate immune system and can contribute to the inflammatory state of visceral adipose tissue. Therefore, it is important to determine the role that omentin plays in the regulation of chronic inflammation. Primary cultures of human adipocyte mesothelial cells were isolated by biopsy trypsinization and Immunofluorescence microscopy was used to determine the purity of primary HAMC cultures. To determine if chronic inflammation is the cause of decreased omentin expression in obesity, HAMCs were treated with TNF-alpha, a pro-inflammatory cytokine, for 24 hours to simulate chronic exposure. Omentin gene expression was down-regulated by TNF-alpha significantly (39% decrease) in serum-free chronic conditions. The protein expression of omentin was also significantly decreased (31%) in serum containing conditions. To demonstrate that omentin's insulin-sensitizing activity may be mediated by anti-inflammatory actions, inflammation-dependent NF-kB luciferase reporter assays were performed in 3T3-L1 adipocytes and human adipose mesothelial cells treated with omentin and/or TNF-alpha. Omentin decreased pro-inflammatory gene transcription in 3T3-L1 adipocytes (85% decrease) and in HAMCs (68% decrease). In order to ascertain the role that omentin plays in the regulation cytokine secretion and what would result if omentin were not present, lentivirus containing omentin-specific shRNAs were generated. A complete knockdown was not achieved, but stimulation with LPS significantly increased (1.73%) the amount of TNF-alpha secretion in mesothelial cells suggesting that even partially reduced omentin protein expression can have a profound impact on the mesothelial inflammatory response. In conclusion, omentin plays a role in the anti-inflammatory response, but can also be negatively regulated by excessive and chronic pro-inflammatory cytokines.
    Description
    University of Maryland, Baltimore. Biomedical Research. M.S. 2013
    Keyword
    mesothelial cells
    Adipose Tissue
    Inflammation
    Obesity
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/2754
    Collections
    Theses and Dissertations All Schools
    Theses and Dissertations School of Medicine

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.