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    A soft-tissue in vitro model of bisphosphonate induced localized non-traditional calciphylaxis

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    Author
    Alfouzan, Weam
    Advisor
    Meiller, Timothy F.
    Date
    2013
    Type
    dissertation
    
    Metadata
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    Abstract
    Background: Bisphosphonates (BPs) such as zoledronic acid (ZA) are widely used to treat complications of bony metastases in cancer patients. One of the serious adverse effects of ZA treatment is Bisphosphonates osteonecrosis (BON). The pathophysiologic mechanisms of BON are not fully elucidated. Scheper et al (2012) suggest that BON is correlated with a localized non-traditional calciphylaxis. In this project, we studied the effect of ZA on soft tissue by calcium deposition. Also, we hypothesized that albumin will reverse that effect. Materials and Methods: Normal Oral Keratinocytes (NOK) and Human Gingival Fibroblats (HGF) were treated by different ZA concentration and Bovine Serum Albumin (BSA). Immunoflurosence and Western Blot were used to study the treatments effects. Results: There is an increase in calcium concentration when cells were treated with ZA. However, there is a decrease when cells were treated with albumin. Conclusion: Albumin can bind to the calcium released following ZA treatment. Our finding might suggest a method of BON prevention. Further studies are needed to determine if such effects are clinically relevant for in vivo models of BON.
    Description
    University of Maryland, Baltimore. Biomedical Science. M.S. 2013
    Keyword
    bisphosphonate osteonecrosis--prevention
    Bisphosphonate-Associated Osteonecrosis of the Jaw--prevention & control
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/2743
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