• Login
    View Item 
    •   UMB Digital Archive
    • School, Graduate
    • Theses and Dissertations All Schools
    • View Item
    •   UMB Digital Archive
    • School, Graduate
    • Theses and Dissertations All Schools
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Identification of intracellular calcium pools and calcium translocation mechanisms in DDT(1)MF-2 smooth muscle cells

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Find Full text
    Author
    Bian, Junhui
    Advisor
    Gill, Donald L.
    Date
    1992
    Type
    dissertation
    
    Metadata
    Show full item record
    Abstract
    The focus of the present studies is on the functional characterization of discrete Ca{dollar}\sp{lcub}2+{rcub}{dollar} pools on the basis of subcellular location, Ca{dollar}\sp{lcub}2+{rcub}{dollar} transport properties, and functional roles. In both saponin-permeabilized DDT{dollar}\sb1{dollar}MF-2 smooth muscle cells and purified rough endoplasmic reticulum (ER) vesicles from these cells, sphingosine derivatives, in particular, sphingosine (Sph) and sphingosyl-phosphorylcholine (SPC) were observed to induce rapid and profound intracellular Ca{dollar}\sp{lcub}2+{rcub}{dollar} release as revealed by {dollar}\sp{lcub}45{rcub}{dollar}Ca{dollar}\sp{lcub}2+{rcub}{dollar} uptake and release assays. The properties of sphingoid base-induced Ca{dollar}\sp{lcub}2+{rcub}{dollar} release were found highly comparable to their reported effects on PKC inhibition in terms of sensitivity, molecular specificity and reversibility. Sphingoid bases released Ca{dollar}\sp{lcub}2+{rcub}{dollar} up to the same level of the combined action of inositol-1,4,5-triphosphate (InsP{dollar}\sb3){dollar} and GTP, and no further Ca{dollar}\sp{lcub}2+{rcub}{dollar} release induced by InsP{dollar}\sb3{dollar} together with GTP was seen after maximal effect of sphingosine derivatives, indicating a shared Ca{dollar}\sp{lcub}2+{rcub}{dollar} pool target with InsP{dollar}\sb3{dollar} and GTP. The action of Sph, in contrast to that of SPC, was blocked by ADP (100 {dollar}\mu{dollar}M) addition, ATP depletion and low temperature, indicating that Sph had no intrinsic activity in releasing Ca{dollar}\sp{lcub}2+{rcub}{dollar} and must be converted into an active form. The results indicate that generation of sphingoid bases in cells may activate a dual signaling pathway involving Ca{dollar}\sp{lcub}2+{rcub}{dollar} release in parallel to PKC inhibition. Thapsigargin (TG) inhibited 75% of total intracellular ATP-dependent Ca{dollar}\sp{lcub}2+{rcub}{dollar} uptake with an IC{dollar}\sb{lcub}50{rcub}{dollar} of 30 nM. TG-sensitive Ca{dollar}\sp{lcub}2+{rcub}{dollar} pumps exist in two previously defined pools--the InsP{dollar}\sb3{dollar}-sensitive, oxalate-permeable Ca{dollar}\sp{lcub}2+{rcub}{dollar} pool, and the InsP{dollar}\sb3{dollar}-insensitive, oxalate-impermeable Ca{dollar}\sp{lcub}2+{rcub}{dollar} pool. A brief TG treatment of 30 min caused persistent Ca{dollar}\sp{lcub}2+{rcub}{dollar} depletion in TG-sensitive pools as well as total arrest of cell growth. A precise correlation between the Ca{dollar}\sp{lcub}2+{rcub}{dollar} content in signaling pools and cell growth was shown to exist. (Abstract shortened by UMI.)
    Description
    University of Maryland, Baltimore. Biochemistry and Molecular Biology. Ph.D. 1992
    Keyword
    Biology, Cell
    calcium pools
    DDT(1)MF-2 smooth muscle cells
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/2523
    Collections
    Theses and Dissertations All Schools
    Theses and Dissertations School of Medicine

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.