Comparison of Two Different Blood Incubation and Monitoring Systems
Abstract
Background: Management of bloodstream infections requires fast and accurate diagnostic testing to better patient outcomes. Blood culture incubation and monitoring systems are critical for identifying positive blood cultures in a timely matter. Reducing incubation time can reduce time to results. Methods: This study compared two blood culture incubation systems and their media, BD BACTEC™ Plus Aerobic/F media (Becton Dickinson) and BacT/Alert®3D FA plus (BioMérieux) to determine their time to detection and recovery rate. In triplicate, 50 matched bacterial strains were inoculated at 10-100 CFU per 0.1ml and incubated on each instrument. Time to positivity was recorded and difference in time to positivity between matched strains was calculated. Wilcoxon signed- rank test was used to calculate significance. Results: All blood cultures were positive within 24 hours. The median time to positivity for all strains was 12 hours and 30 minutes (Interquartile range (IQR): 11:08, 15:55) for BacT/Alert®3D and 11 hours and 13 minutes for BACTEC™ FX (IQR: 10:05, 14:52) (p <0.001) with average difference in time to positivity being 54.6 minutes. For Gram-positive bacteria, median time to positivity was 13 hours and 54 minutes (IQR: 11:44, 16:18) for BacT/Alert®3D and 12 hours and 29 minutes for the BACTEC™ FX (IQR: 10:53, 16:05) (p = 0.02) with an average difference in time to positivity of 57.3 minutes. For Gram- negative bacteria, median time to positivity was 11 hours and 20 minutes (IQR: 10:52, 12:01) for BacT/Alert®3D and 10 hours and 23 minutes for BACTEC™ FX (IQR: 9:37, 11:14) (p < 0.001). Average difference in time to positivity was 61.1 minutes. For fastidious organisms, the median time was 18 hours and 46 minutes for BacT/Alert®3D and 13 hours and 44 minutes for BACTEC™ FX. Conclusions: BACTEC™ FX significantly reduced time to positivity by approximately one hour when compared to BacT/Alert®3D. This reduction in time could improve patient outcomesDescription
American Society for Microbiology. June 10, 2024.Rights/Terms
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http://hdl.handle.net/10713/22576Collections
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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International