Show simple item record

dc.contributor.authorBlackwood, Catherine B
dc.contributor.authorMateu-Borrás, Margalida
dc.contributor.authorSen-Kilic, Emel
dc.contributor.authorPyles, Gage M
dc.contributor.authorMiller, Sarah Jo
dc.contributor.authorWeaver, Kelly L
dc.contributor.authorWitt, William T
dc.contributor.authorHuckaby, Annalisa B
dc.contributor.authorKang, Jason
dc.contributor.authorChandler, Courtney E
dc.contributor.authorErnst, Robert K
dc.contributor.authorHeath Damron, F
dc.contributor.authorBarbier, Mariette
dc.date.accessioned2022-11-22T19:23:04Z
dc.date.available2022-11-22T19:23:04Z
dc.date.issued2022-11-10
dc.identifier.urihttp://hdl.handle.net/10713/20213
dc.description.abstractWhole cell vaccines are complex mixtures of antigens, immunogens, and sometimes adjuvants that can trigger potent and protective immune responses. In some instances, such as whole cell Bordetella pertussis vaccination, the immune response to vaccination extends beyond the pathogen the vaccine was intended for and contributes to protection against other clinically significant pathogens. In this study, we describe how B. pertussis whole cell vaccination protects mice against acute pneumonia caused by Pseudomonas aeruginosa. Using ELISA and western blot, we identified that B. pertussis whole cell vaccination induces production of antibodies that bind to lab-adapted and clinical strains of P. aeruginosa, regardless of immunization route or adjuvant used. The cross-reactive antigens were identified using immunoprecipitation, mass spectrometry, and subsequent immunoblotting. We determined that B. pertussis GroEL and OmpA present in the B. pertussis whole cell vaccine led to production of antibodies against P. aeruginosa GroEL and OprF, respectively. Finally, we showed that recombinant B. pertussis OmpA was sufficient to induce protection against P. aeruginosa acute murine pneumonia. This study highlights the potential for use of B. pertussis OmpA as a vaccine antigen for prevention of P. aeruginosa infection, and the potential of broadly protective antigens for vaccine development.en_US
dc.description.urihttps://doi.org/10.1038/s41541-022-00562-1en_US
dc.language.isoenen_US
dc.relation.ispartofNPJ vaccinesen_US
dc.rights© 2022. The Author(s).en_US
dc.titleBordetella pertussis whole cell immunization protects against Pseudomonas aeruginosa infections.en_US
dc.typeArticleen_US
dc.identifier.doi10.1038/s41541-022-00562-1
dc.identifier.pmid36357402
dc.source.journaltitleNPJ vaccines
dc.source.volume7
dc.source.issue1
dc.source.beginpage143
dc.source.endpage
dc.source.countryUnited States
dc.source.countryEngland


This item appears in the following Collection(s)

Show simple item record