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    Metabolism of Exogenous [2,4-13C]β-Hydroxybutyrate following Traumatic Brain Injury in 21-22-Day-Old Rats: An Ex Vivo NMR Study

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    Author
    Scafidi, Susanna
    Jernberg, Jennifer
    Fiskum, Gary
    McKenna, Mary C.
    Date
    2022-08-01
    Journal
    Metabolites
    Publisher
    MDPI
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.3390/metabo12080710
    Abstract
    Traumatic brain injury (TBI) is leading cause of morbidity in young children. Acute dysregulation of oxidative glucose metabolism within the first hours after injury is a hallmark of TBI. The developing brain relies on ketones as well as glucose for energy. Thus, the aim of this study was to determine the metabolism of ketones early after TBI injury in the developing brain. Following the controlled cortical impact injury model of TBI, 21-22-day-old rats were infused with [2,4-13C]β-hydroxybutyrate during the acute (4 h) period after injury. Using ex vivo 13C-NMR spectroscopy, we determined that 13C-β-hydroxybutyrate (13C-BHB) metabolism was increased in both the ipsilateral and contralateral sides of the brain after TBI. Incorporation of the label was significantly higher in glutamate than glutamine, indicating that 13C-BHB metabolism was higher in neurons than astrocytes in both sham and injured brains. Our results show that (i) ketone metabolism was significantly higher in both the ipsilateral and contralateral sides of the injured brain after TBI; (ii) ketones were extensively metabolized by both astrocytes and neurons, albeit higher in neurons; (iii) the pyruvate recycling pathway determined by incorporation of the label from the metabolism of 13C-BHB into lactate was upregulated in the immature brain after TBI.
    Description
    Data are contained within the article.
    Sponsors
    National Institute of Neurological Disorders and Stroke
    Keyword
    developing brain
    ketone bodies
    metabolism
    traumatic brain injury
    β-hydroxybutyrate
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/19801
    ae974a485f413a2113503eed53cd6c53
    10.3390/metabo12080710
    Scopus Count
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