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dc.contributor.authorDai, Li
dc.contributor.authorUehara, Mayuko
dc.contributor.authorLi, Xiaofei
dc.contributor.authorLaBarre, Brenna A
dc.contributor.authorBanouni, Naima
dc.contributor.authorIchimura, Takaharu
dc.contributor.authorLee-Sundlov, Melissa M
dc.contributor.authorKasinath, Vivek
dc.contributor.authorSullivan, Jade A
dc.contributor.authorNi, Heyu
dc.contributor.authorBarone, Francesca
dc.contributor.authorGiannini, Silvia
dc.contributor.authorBahmani, Baharak
dc.contributor.authorSage, Peter T
dc.contributor.authorPatsopoulos, Nikolaos A
dc.contributor.authorTsokos, George C
dc.contributor.authorBromberg, Jonathan S
dc.contributor.authorHoffmeister, Karin
dc.contributor.authorJiang, Liwei
dc.contributor.authorAbdi, Reza
dc.date.accessioned2022-08-31T17:28:49Z
dc.date.available2022-08-31T17:28:49Z
dc.date.issued2022-08-11
dc.identifier.urihttp://hdl.handle.net/10713/19647
dc.description.abstractLymph nodes (LNs) are the critical sites of immunity, and the stromal cells of LNs are crucial to their function. Our understanding of the stromal compartment of the LN has deepened recently with the characterization of nontraditional stromal cells. CD41 (integrin αIIb) is known to be expressed by platelets and hematolymphoid cells. We identified two distinct populations of CD41+Lyve1+ and CD41+Lyve1- cells in the LNs. CD41+Lyve1- cells appear in the LN mostly at the later stages of the lives of mice. We identified CD41+ cells in human LNs as well. We demonstrated that murine CD41+ cells express mesodermal markers, such as Sca-1, CD105 and CD29, but lack platelet markers. We did not observe the presence of platelets around the HEVs or within proximity to fibroblastic reticular cells of the LN. Examination of thoracic duct lymph fluid showed the presence of CD41+Lyve1- cells, suggesting that these cells recirculate throughout the body. FTY720 reduced their trafficking to lymph fluid, suggesting that their egress is controlled by the S1P1 pathway. CD41+Lyve1- cells of the LNs were sensitive to radiation, suggestive of their replicative nature. Single cell RNA sequencing data showed that the CD41+ cell population in naïve mouse LNs expressed largely stromal cell markers. Further studies are required to examine more deeply the role of CD41+ cells in the function of LNs.en_US
dc.description.urihttps://doi.org/10.3389/fimmu.2022.801945en_US
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.relation.ispartofFrontiers in Immunologyen_US
dc.rightsCopyright © 2022 Dai, Uehara, Li, LaBarre, Banouni, Ichimura, Lee-Sundlov, Kasinath, Sullivan, Ni, Barone, Giannini, Bahmani, Sage, Patsopoulos, Tsokos, Bromberg, Hoffmeister, Jiang and Abdi.en_US
dc.subjectCD41 + progenitor cellsen_US
dc.subjectlymph nodesen_US
dc.subjectlymphaticen_US
dc.subjectlymphatic remodelingen_US
dc.subjectstromal cellsen_US
dc.titleCharacterization of CD41 cells in the lymph node.en_US
dc.typeArticleen_US
dc.identifier.doi10.3389/fimmu.2022.801945
dc.identifier.pmid36032128
dc.source.journaltitleFrontiers in immunology
dc.source.volume13
dc.source.beginpage801945
dc.source.endpage
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countrySwitzerland


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