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dc.contributor.authorNyblade, Charlotte
dc.contributor.authorParreno, Viviana
dc.contributor.authorZhou, Peng
dc.contributor.authorHensley, Casey
dc.contributor.authorOakes, Vanessa
dc.contributor.authorMahsoub, Hassan M
dc.contributor.authorKiley, Kelsey
dc.contributor.authorFrazier, Maggie
dc.contributor.authorFrazier, Annie
dc.contributor.authorZhang, Yongrong
dc.contributor.authorFeng, Hanping
dc.contributor.authorYuan, Lijuan
dc.date.accessioned2022-06-10T12:02:01Z
dc.date.available2022-06-10T12:02:01Z
dc.date.issued2022-06-06
dc.identifier.urihttp://hdl.handle.net/10713/19108
dc.description.abstractClostridioides difficile (C. difficile) is a gram-positive, spore-forming, anaerobic bacterium known to be the most common cause of hospital-acquired and antibiotic-associated diarrhea. C. difficile infection rates are on the rise worldwide and treatment options are limited, indicating a clear need for novel therapeutics. Gnotobiotic piglets are an excellent model to reproduce the acute pseudomembranous colitis (PMC) caused by C. difficile due to their physiological similarities to humans and high susceptibility to infection. Here, we established a gnotobiotic pig model of C. difficile infection and disease using a hypervirulent strain. C. difficile-infected pigs displayed classic signs of C. difficile infection, including severe diarrhea and weight loss. Inoculated pigs had severe gross and microscopic intestinal lesions. C. difficile infection caused an increase in pro-inflammatory cytokines in samples of serum, large intestinal contents, and pleural effusion. C. difficile spores and toxins were detected in the feces of inoculated animals as tested by anaerobic culture and cytotoxicity assays. Successful establishment of this model is key for future work as therapeutics can be evaluated in an environment that accurately mimics what happens in humans. The model is especially suitable for evaluating potential prophylactics and therapeutics, including vaccines and passive immune strategies.en_US
dc.description.urihttps://doi.org/10.1186/s13099-022-00496-yen_US
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.relationAll data are included in this manuscript.en_US
dc.relation.ispartofGut Pathogensen_US
dc.rights© 2022. The Author(s).en_US
dc.subjectClostridioides difficile infection/illness (CDI)en_US
dc.subjectGnotobiotic pigsen_US
dc.subjectPseudomembranous colitis (PMC)en_US
dc.titleEstablishment of a gnotobiotic pig model of Clostridioides difficile infection and disease.en_US
dc.typeArticleen_US
dc.identifier.doi10.1186/s13099-022-00496-y
dc.identifier.pmid35668452
dc.source.journaltitleGut pathogens
dc.source.volume14
dc.source.issue1
dc.source.beginpage22
dc.source.endpage
dc.source.countryEngland


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