Caulobacter lipid A is conditionally dispensable in the absence of fur and in the presence of anionic sphingolipids.
AuthorZik, Justin J
Yoon, Sung Hwan
Stankeviciute Skidmore, Gabriele
Gudoor, Ridhi R
Davies, Karen M
Deutschbauer, Adam M
Goodlett, David R
Klein, Eric A
Ryan, Kathleen R
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AbstractLipid A, the membrane-anchored portion of lipopolysaccharide (LPS), is an essential component of the outer membrane (OM) of nearly all Gram-negative bacteria. Here we identify regulatory and structural factors that together render lipid A nonessential in Caulobacter crescentus. Mutations in the ferric uptake regulator fur allow Caulobacter to survive in the absence of either LpxC, which catalyzes an early step of lipid A synthesis, or CtpA, a tyrosine phosphatase homolog we find is needed for wild-type lipid A structure and abundance. Alterations in Fur-regulated processes, rather than iron status per se, underlie the ability to survive when lipid A synthesis is blocked. Fitness of lipid A-deficient Caulobacter requires an anionic sphingolipid, ceramide phosphoglycerate (CPG), which also mediates sensitivity to the antibiotic colistin. Our results demonstrate that, in an altered regulatory landscape, anionic sphingolipids can support the integrity of a lipid A-deficient OM.
Data AvailibilityThis paper analyzes existing, publicly available data, whose sources are listed in the key resources table.
Rights/TermsCopyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
Identifier to cite or link to this itemhttp://hdl.handle.net/10713/19080