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dc.contributor.authorZhen, Yilan
dc.contributor.authorCullen, Carlie L
dc.contributor.authorRicci, Raphael
dc.contributor.authorSummers, Benjamin S
dc.contributor.authorRehman, Sakina
dc.contributor.authorAhmed, Zubair M
dc.contributor.authorFoster, Antoinette Y
dc.contributor.authorEmery, Ben
dc.contributor.authorGasperini, Robert
dc.contributor.authorYoung, Kaylene M
dc.date.accessioned2022-06-02T14:05:56Z
dc.date.available2022-06-02T14:05:56Z
dc.date.issued2022-05-30
dc.identifier.urihttp://hdl.handle.net/10713/19048
dc.description.sponsorshipOligodendrocyte progenitor cells (OPCs) express protocadherin 15 (Pcdh15), a member of the cadherin superfamily of transmembrane proteins. Little is known about the function of Pcdh15 in the central nervous system (CNS), however, Pcdh15 expression can predict glioma aggression and promote the separation of embryonic human OPCs immediately following a cell division. Herein, we show that Pcdh15 knockdown significantly increases extracellular signal-related kinase (ERK) phosphorylation and activation to enhance OPC proliferation in vitro. Furthermore, Pcdh15 knockdown elevates Cdc42-Arp2/3 signalling and impairs actin kinetics, reducing the frequency of lamellipodial extrusion and slowing filopodial withdrawal. Pcdh15 knockdown also reduces the number of processes supported by each OPC and new process generation. Our data indicate that Pcdh15 is a critical regulator of OPC proliferation and process motility, behaviours that characterise the function of these cells in the healthy CNS, and provide mechanistic insight into the role that Pcdh15 might play in glioma progression.en_US
dc.description.urihttps://doi.org/10.1038/s42003-022-03470-1en_US
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.relationThe associated raw video and image files are a research resource that will be made available by the corresponding author, upon reasonable request.en_US
dc.relation.ispartofCommunications Biologyen_US
dc.rights© 2022. The Author(s).en_US
dc.titleProtocadherin 15 suppresses oligodendrocyte progenitor cell proliferation and promotes motility through distinct signalling pathways.en_US
dc.typeArticleen_US
dc.identifier.doi10.1038/s42003-022-03470-1
dc.identifier.pmid35637313
dc.source.journaltitleCommunications biology
dc.source.volume5
dc.source.issue1
dc.source.beginpage511
dc.source.endpage
dc.source.countryEngland


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