Deuterated Linoleic Acid Attenuates the RBC Storage Lesion in a Mouse Model of Poor RBC Storage.
AuthorKim, Christopher Y
Spitalnik, Steven L
Hod, Eldad A
Francis, Richard O
Hudson, Krystalyn E
Stone, Elizabeth F
Gordy, Dominique E
Zimring, James C
Buehler, Paul W
Wilson, Robert B
Shchepinov, Mikhail S
JournalFrontiers in Physiology
PublisherFrontiers Media S.A.
MetadataShow full item record
AbstractBackground: Long-chain polyunsaturated fatty acids (PUFAs) are important modulators of red blood cell (RBC) rheology. Dietary PUFAs are readily incorporated into the RBC membrane, improving RBC deformability, fluidity, and hydration. However, enriching the lipid membrane with PUFAs increases the potential for peroxidation in oxidative environments (e.g., refrigerated storage), resulting in membrane damage. Substitution of bis-allylic hydrogens with deuterium ions in PUFAs decreases hydrogen abstraction, thereby inhibiting peroxidation. If lipid peroxidation is a causal factor in the RBC storage lesion, incorporation of deuterated linoleic acid (DLA) into the RBC membrane should decrease lipid peroxidation, thereby improving RBC lifespan, deformability, filterability, and post-transfusion recovery (PTR) after cold storage. Study Design and Methods: Mice associated with good (C57BL/6J) and poor (FVB) RBC storage quality received diets containing 11,11-D2-LA Ethyl Ester (1.0 g/100 g diet; deuterated linoleic acid) or non-deuterated LA Ethyl Ester (control) for 8 weeks. Deformability, filterability, lipidomics, and lipid peroxidation markers were evaluated in fresh and stored RBCs. Results: DLA was incorporated into RBC membranes in both mouse strains. DLA diet decreased lipid peroxidation (malondialdehyde) by 25.4 and 31% percent in C57 mice and 12.9 and 79.9% in FVB mice before and after cold storage, respectively. In FVB, but not C57 mice, deformability filterability, and post-transfusion recovery were significantly improved. Discussion: In a mouse model of poor RBC storage, with elevated reactive oxygen species production, DLA attenuated lipid peroxidation and significantly improved RBC storage quality.
Rights/TermsCopyright © 2022 Kim, Johnson, Peltier, Spitalnik, Hod, Francis, Hudson, Stone, Gordy, Fu, Zimring, Amireault, Buehler, Wilson, D’Alessandro, Shchepinov and Thomas.
Identifier to cite or link to this itemhttp://hdl.handle.net/10713/18872
- Isotope-reinforced polyunsaturated fatty acids protect mitochondria from oxidative stress.
- Authors: Andreyev AY, Tsui HS, Milne GL, Shmanai VV, Bekish AV, Fomich MA, Pham MN, Nong Y, Murphy AN, Clarke CF, Shchepinov MS
- Issue date: 2015 May
- Insights into the role of oxidative stress in the pathology of Friedreich ataxia using peroxidation resistant polyunsaturated fatty acids.
- Authors: Cotticelli MG, Crabbe AM, Wilson RB, Shchepinov MS
- Issue date: 2013
- Threshold protective effect of deuterated polyunsaturated fatty acids on peroxidation of lipid bilayers.
- Authors: Firsov AM, Fomich MA, Bekish AV, Sharko OL, Kotova EA, Saal HJ, Vidovic D, Shmanai VV, Pratt DA, Antonenko YN, Shchepinov MS
- Issue date: 2019 Jun
- Strain-specific red blood cell storage, metabolism, and eicosanoid generation in a mouse model.
- Authors: Zimring JC, Smith N, Stowell SR, Johnsen JM, Bell LN, Francis RO, Hod EA, Hendrickson JE, Roback JD, Spitalnik SL
- Issue date: 2014 Jan