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dc.contributor.authorMa, Jie
dc.contributor.authorLi, Ning
dc.contributor.authorGuarnera, Maria
dc.contributor.authorJiang, Feng
dc.date.accessioned2022-05-04T12:44:29Z
dc.date.available2022-05-04T12:44:29Z
dc.date.issued2013-11-14
dc.identifier.urihttp://hdl.handle.net/10713/18749
dc.description.abstractotential approach for cancer diagnosis. However, absolutely quantifying low abundant plasma miRNAs is challenging with qPCR. Digital PCR offers a unique means for assessment of nucleic acids presenting at low levels in plasma. This study aimed to evaluate the efficacy of digital PCR for quantification of plasma miRNAs and the potential utility of this technique for cancer diagnosis. We used digital PCR to quantify the copy number of plasma microRNA-21-5p (miR-21–5p) and microRNA-335–3p (miR-335–3p) in 36 lung cancer patients and 38 controls. Digital PCR showed a high degree of linearity and quantitative correlation with miRNAs in a dynamic range from 1 to 10,000 copies/μL of input, with high reproducibility. qPCR exhibited a dynamic range from 100 to 1X107 copies/μL of input. Digital PCR had a higher sensitivity to detect copy number of the miRNAs compared with qPCR. In plasma, digital PCR could detect copy number of both miR-21–5p and miR-335–3p, whereas qPCR was only able to assess miR-21–5p. Quantification of the plasma miRNAs by digital PCR provided 71.8% sensitivity and 80.6% specificity in distinguishing lung cancer patients from cancer-free subjects.en_US
dc.description.urihttps://doi.org/10.4137/BMI.S13154en_US
dc.language.isoenen_US
dc.publisherSAGE Publications Inc.en_US
dc.relation.ispartofBiomarker Insightsen_US
dc.subjectdiagnosisen_US
dc.subjectdigital PCRen_US
dc.subjectlung canceren_US
dc.subjectmiRNAsen_US
dc.subjectplasmaen_US
dc.titleQuantification of Plasma miRNAs by Digital PCR for Cancer Diagnosis.en_US
dc.typeArticleen_US
dc.identifier.doi10.4137/BMI.S13154
dc.identifier.pmid24277982
dc.source.journaltitleBiomarker insights
dc.source.volume8
dc.source.beginpage127
dc.source.endpage36
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States


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