Dopamine-induced pruning in monocyte-derived-neuronal-like cells (MDNCs) from patients with schizophrenia.
Author
Bellon, AlfredoFeuillet, Vincent
Cortez-Resendiz, Alonso
Mouaffak, Faycal
Kong, Lan
Hong, L Elliot
De Godoy, Lilian
Jay, Therese M
Hosmalin, Anne
Krebs, Marie-Odile
Date
2022-04-01Journal
Molecular PsychiatryPublisher
Springer NatureType
Article
Metadata
Show full item recordAbstract
The long lapse between the presumptive origin of schizophrenia (SCZ) during early development and its diagnosis in late adolescence has hindered the study of crucial neurodevelopmental processes directly in living patients. Dopamine, a neurotransmitter consistently associated with the pathophysiology of SCZ, participates in several aspects of brain development including pruning of neuronal extensions. Excessive pruning is considered the cause of the most consistent finding in SCZ, namely decreased brain volume. It is therefore possible that patients with SCZ carry an increased susceptibility to dopamine’s pruning effects and that this susceptibility would be more obvious in the early stages of neuronal development when dopamine pruning effects appear to be more prominent. Obtaining developing neurons from living patients is not feasible. Instead, we used Monocyte-Derived-Neuronal-like Cells (MDNCs) as these cells can be generated in only 20 days and deliver reproducible results. In this study, we expanded the number of individuals in whom we tested the reproducibility of MDNCs. We also deepened the characterization of MDNCs by comparing its neurostructure to that of human developing neurons. Moreover, we studied MDNCs from 12 controls and 13 patients with SCZ. Patients’ cells differentiate more efficiently, extend longer secondary neurites and grow more primary neurites. In addition, MDNCs from medicated patients expresses less D1R and prune more primary neurites when exposed to dopamine. Haloperidol did not influence our results but the role of other antipsychotics was not examined and thus, needs to be considered as a confounder. © 2022, The Author(s).Rights/Terms
© 2022. The Author(s).Identifier to cite or link to this item
http://hdl.handle.net/10713/18573ae974a485f413a2113503eed53cd6c53
10.1038/s41380-022-01514-w
Scopus Count
Collections
Related articles
- Optimization of Neurite Tracing and Further Characterization of Human Monocyte-Derived-Neuronal-like Cells.
- Authors: Bellon A, Hasoglu T, Peterson M, Gao K, Chen M, Blandin E, Cortez-Resendiz A, Clawson GA, Hong LE
- Issue date: 2021 Oct 20
- Transdifferentiation of Human Circulating Monocytes Into Neuronal-Like Cells in 20 Days and Without Reprograming.
- Authors: Bellon A, Wegener A, Lescallette AR, Valente M, Yang SK, Gardette R, Matricon J, Mouaffak F, Watts P, Vimeux L, Yun JK, Kawasawa YI, Clawson GA, Blandin E, Chaumette B, Jay TM, Krebs MO, Feuillet V, Hosmalin A
- Issue date: 2018
- Abnormal synaptic pruning during adolescence underlying the development of psychotic disorders.
- Authors: Germann M, Brederoo SG, Sommer IEC
- Issue date: 2021 May 1
- Impact of nuclear distribution element genes in the typical and atypical antipsychotics effects on nematode Caenorhabditis elegans: Putative animal model for studying the pathways correlated to schizophrenia.
- Authors: Monte GG, Nani JV, de Almeida Campos MR, Dal Mas C, Marins LAN, Martins LG, Tasic L, Mori MA, Hayashi MAF
- Issue date: 2019 Jun 8
- Microglia Activation and Schizophrenia: Lessons From the Effects of Minocycline on Postnatal Neurogenesis, Neuronal Survival and Synaptic Pruning.
- Authors: Inta D, Lang UE, Borgwardt S, Meyer-Lindenberg A, Gass P
- Issue date: 2017 May 1