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dc.contributor.authorGe, Zhi Dong
dc.contributor.authorBoyd, Riley M.
dc.contributor.authorLantz, Connor
dc.contributor.authorThorp, Edward B.
dc.contributor.authorForbess, Joseph M.
dc.date.accessioned2022-04-14T14:34:34Z
dc.date.available2022-04-14T14:34:34Z
dc.date.issued2022-01-01
dc.identifier.urihttp://hdl.handle.net/10713/18567
dc.description.abstractObjective: The pedicled greater omentum, when applied onto stressed hearts using omentopexy, has been shown to be protective in humans and animals. The mechanisms underlying cardioprotection using omentopexy remain elusive. This study examined whether macrophage-mediated angiogenesis accounts for the cardioprotective effect of omentopexy in mice. Methods: C57BL/6 mice were subjected to minimally invasive transverse aortic constriction for 6 weeks and subsequent cardio-omentopexy for 8 weeks. Control mice underwent the same surgical procedures without aortic constriction or cardio-omentopexy. Results: Transverse aortic constriction led to left ventricular concentric hypertrophy, reduced mitral E/A ratio, increased cardiomyocyte size, and myocardial fibrosis in the mice that underwent sham cardio-omentopexy surgery. The negative effects of transverse aortic constriction were prevented by cardio-omentopexy. Myocardial microvessel density was elevated in the mice that underwent aortic constriction and sham cardio-omentopexy surgery, and cardio-omentopexy further enhanced angiogenesis. Nanostring gene array analysis uncovered the activation of angiogenesis gene networks by cardio-omentopexy. Flow cytometric analysis revealed that cardio-omentopexy triggered the accumulation of cardiac MHCIIloLyve1+TimD4+ (Major histocompatibility complex class IIlow lymphatic vessel endothelial hyaluronan receptor 1+ T cell immunoglobulin and mucin domain conataining 4+) resident macrophages at the omental–cardiac interface. Intriguingly, the depletion of macrophages with clodronate-liposome resulted in the failure of cardio-omentopexy to protect the heart and promote angiogenesis. Conclusions: Cardio-omentopexy protects the heart from pressure overload-elicited left ventricular hypertrophy and dysfunction by promoting myocardial angiogenesis. Cardiac MHCIIloLyve1+TimD4+ resident macrophages play a critical role in the cardioprotective effect and angiogenesis of cardio-omentopexy. Video Abstract: © 2022 The Author(s)en_US
dc.description.sponsorshipNational Institutes of Healthen_US
dc.description.urihttps://doi.org/10.1016/j.xjon.2022.02.027en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJTCVS Openen_US
dc.subjectangiogenesisen_US
dc.subjectcardiac hypertrophyen_US
dc.subjectcardio-omentopexyen_US
dc.subjectmacrophagesen_US
dc.titleCardio-omentopexy requires a cardioprotective innate immune response to promote myocardial angiogenesis in miceen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.xjon.2022.02.027
dc.source.journaltitleJTCVS Open


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