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    Cardio-omentopexy requires a cardioprotective innate immune response to promote myocardial angiogenesis in mice

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    Author
    Ge, Zhi Dong
    Boyd, Riley M.
    Lantz, Connor
    Thorp, Edward B.
    Forbess, Joseph M.
    Date
    2022-01-01
    Journal
    JTCVS Open
    Publisher
    Elsevier
    Type
    Article
    
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    See at
    https://doi.org/10.1016/j.xjon.2022.02.027
    Abstract
    Objective: The pedicled greater omentum, when applied onto stressed hearts using omentopexy, has been shown to be protective in humans and animals. The mechanisms underlying cardioprotection using omentopexy remain elusive. This study examined whether macrophage-mediated angiogenesis accounts for the cardioprotective effect of omentopexy in mice. Methods: C57BL/6 mice were subjected to minimally invasive transverse aortic constriction for 6 weeks and subsequent cardio-omentopexy for 8 weeks. Control mice underwent the same surgical procedures without aortic constriction or cardio-omentopexy. Results: Transverse aortic constriction led to left ventricular concentric hypertrophy, reduced mitral E/A ratio, increased cardiomyocyte size, and myocardial fibrosis in the mice that underwent sham cardio-omentopexy surgery. The negative effects of transverse aortic constriction were prevented by cardio-omentopexy. Myocardial microvessel density was elevated in the mice that underwent aortic constriction and sham cardio-omentopexy surgery, and cardio-omentopexy further enhanced angiogenesis. Nanostring gene array analysis uncovered the activation of angiogenesis gene networks by cardio-omentopexy. Flow cytometric analysis revealed that cardio-omentopexy triggered the accumulation of cardiac MHCIIloLyve1+TimD4+ (Major histocompatibility complex class IIlow lymphatic vessel endothelial hyaluronan receptor 1+ T cell immunoglobulin and mucin domain conataining 4+) resident macrophages at the omental–cardiac interface. Intriguingly, the depletion of macrophages with clodronate-liposome resulted in the failure of cardio-omentopexy to protect the heart and promote angiogenesis. Conclusions: Cardio-omentopexy protects the heart from pressure overload-elicited left ventricular hypertrophy and dysfunction by promoting myocardial angiogenesis. Cardiac MHCIIloLyve1+TimD4+ resident macrophages play a critical role in the cardioprotective effect and angiogenesis of cardio-omentopexy. Video Abstract: © 2022 The Author(s)
    Sponsors
    National Institutes of Health
    Keyword
    angiogenesis
    cardiac hypertrophy
    cardio-omentopexy
    macrophages
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/18567
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.xjon.2022.02.027
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