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dc.contributor.authorDoub, James B
dc.contributor.authorUrish, Ken
dc.contributor.authorLee, Martin
dc.contributor.authorFackler, Joseph
dc.date.accessioned2022-04-08T19:13:56Z
dc.date.available2022-04-08T19:13:56Z
dc.date.issued2022-03-21
dc.identifier.urihttp://hdl.handle.net/10713/18508
dc.description.abstractBackground: Given the specificity of bacteriophage attachment receptors, a single bacterial isolate is currently utilized to match to a bacteriophage therapeutic thereby extrapolating activity to all bacteria in vivo. Obstinately, the main bacteriophage attachment receptor for Staphylococcus aureus is teichoic acid and it is known that this receptor has phenotypic variations in different in vivo environments. Consequently, the aim of this study was to determine if bacteriophage activity is similar across all in vivo prosthetic joint infection environments. Methods: Three patients with prosthetic joint infections who had S. aureus grow from arthrocentesis cultures and at least three deep tissue cultures were analyzed for growth inhibition with a library of 56 bacteriophages RESULTS: Discordant bacteriophage activity was seen across the different in vivo environments. As well bacteriophages with the most robust lytic potential to the arthrocentesis isolates usually did not have activity to all the deep tissues clinical isolates. Conclusion: Variations of bacteriophage activity can occur between the different in vivo clinical environments which is likely secondary to different glycosylation patterns of teichoic acid. Consequently, if discordant activity is present then retreating with bacteriophages that have activity is likely needed for effective, reproducible outcomes.en_US
dc.description.urihttps://doi.org/10.1016/j.ijid.2022.03.022en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Journal of Infectious Diseases : IJIDen_US
dc.rightsCopyright © 2022. Published by Elsevier Ltd.en_US
dc.subjectAttachment receptors, Staphylococcusen_US
dc.subjectBacteriophage therapyen_US
dc.subjectPhenotypic variationen_US
dc.subjectProsthetic joint infectionsen_US
dc.titleImpact of Bacterial Phenotypic Variation with Bacteriophage therapy: A Pilot Study with Prosthetic Joint Infection Isolates.en_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.ijid.2022.03.022
dc.identifier.pmid35331932
dc.source.journaltitleInternational journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
dc.source.countryCanada


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