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dc.contributor.authorJiang, Bin
dc.contributor.authorWhite, Alisa
dc.contributor.authorOu, Wenquan
dc.contributor.authorVan Belleghem, Sarah
dc.contributor.authorStewart, Samantha
dc.contributor.authorShamul, James G.
dc.contributor.authorRahaman, Shaik O.
dc.contributor.authorFisher, John P.
dc.contributor.authorHe, Xiaoming
dc.date.accessioned2022-03-25T12:49:37Z
dc.date.available2022-03-25T12:49:37Z
dc.date.issued2022-01-01
dc.identifier.urihttp://hdl.handle.net/10713/18344
dc.description.abstractThe conventional approach for fabricating polydimethylsiloxane (PDMS) microfluidic devices is a lengthy and inconvenient procedure and may require a clean-room microfabrication facility often not readily available. Furthermore, living cells can't survive the oxygen-plasma and high-temperature-baking treatments required for covalent bonding to assemble multiple PDMS parts into a leak-free device, and it is difficult to disassemble the devices because of the irreversible covalent bonding. As a result, seeding/loading cells into and retrieving cells from the devices are challenging. Here, we discovered that decreasing the curing agent for crosslinking the PDMS prepolymer increases the noncovalent binding energy of the resultant PDMS surfaces without plasma or any other treatment. This enables convenient fabrication of leak-free microfluidic devices by noncovalent binding for various biomedical applications that require high pressure/flow rates and/or long-term cell culture, by simply hand-pressing the PDMS parts without plasma or any other treatment to bind/assemble. With this method, multiple types of cells can be conveniently loaded into specific areas of the PDMS parts before assembly and due to the reversible nature of the noncovalent bonding, the assembled device can be easily disassembled by hand peeling for retrieving cells. Combining with 3D printers that are widely available for making masters to eliminate the need of photolithography, this facile yet rigorous fabrication approach is much faster and more convenient for making PDMS microfluidic devices than the conventional oxygen plasma-baking-based irreversible covalent bonding method. © 2022 The Authorsen_US
dc.description.sponsorshipNational Science Foundationen_US
dc.description.urihttps://doi.org/10.1016/j.bioactmat.2022.02.031en_US
dc.language.isoenen_US
dc.publisherKeAi Communications Co.en_US
dc.relation.ispartofBioactive Materialsen_US
dc.subject3D printingen_US
dc.subjectBinding energyen_US
dc.subjectMicrofluidicsen_US
dc.subjectPolydimethylsiloxaneen_US
dc.subjectSoft lithographyen_US
dc.titleNoncovalent reversible binding-enabled facile fabrication of leak-free PDMS microfluidic devices without plasma treatment for convenient cell loading and retrievalen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.bioactmat.2022.02.031
dc.source.journaltitleBioactive Materials


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