• Login
    View Item 
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Subcellular regulation of glucose metabolism through multienzyme glucosome assemblies by EGF-ERK1/2 signaling pathways.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Publisher version
    View Source
    Access full-text PDFOpen Access
    View Source
    Check access options
    Check access options
    Author
    Jeon, Miji
    Chauhan, Krishna M
    Szeto, Gregory L
    Kyoung, Minjoung
    An, Songon
    Date
    2022-02-02
    Journal
    Journal of Biological Chemistry
    Publisher
    Elsevier
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1016/j.jbc.2022.101675
    Abstract
    A multienzyme metabolic assembly for human glucose metabolism, namely the glucosome, has been previously demonstrated to partition glucose flux between glycolysis and building block biosynthesis in an assembly size-dependent manner. Among three different sizes of glucosome assemblies, we have shown that large-sized glucosomes are functionally associated with the promotion of serine biosynthesis in the presence of epidermal growth factor (EGF). However, due to multifunctional roles of EGF in signaling pathways, it is unclear which EGF-mediated signaling pathways promote these large glucosome assemblies in cancer cells. In this study, we used Luminex multiplexing assays and high-content single-cell imaging to demonstrate that EGF triggers temporal activation of extracellular signal-regulated kinases 1/2 (ERK1/2) in Hs578T cells. Subsequently, we found that treatments with a pharmacological inhibitor of ERK1/2, SCH772984, or short-hairpin RNAs targeting ERK1/2 promote the dissociation of large-sized assemblies to medium-sized assemblies in Hs578T cells. In addition, our Western blot analyses revealed that EGF treatment does not increase the expression levels of enzymes that are involved in both glucose metabolism and serine biosynthesis. The observed spatial transition of glucosome assemblies between large and medium sizes appears to be mediated by the degree of dynamic partitioning of glucosome enzymes without changing their expression levels. Collectively, our study demonstrates that EGF–ERK1/2 signaling pathways play an important role in the upregulation of large-sized glucosomes in cancer cells, thus functionally governing the promotion of glycolysis-derived serine biosynthesis. © 2022 THE AUTHORS.
    Rights/Terms
    Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
    Keyword
    epidermal growth factor (EGF)
    extracellular-signal-regulated kinase (ERK)
    glucose metabolism
    glycolysis
    live-cell imaging
    metabolic condensate
    metabolic regulation
    protein assembly
    serine biosynthesis
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/18224
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.jbc.2022.101675
    Scopus Count
    Collections
    UMB Open Access Articles

    entitlement

    Related articles

    • Multi-dimensional Fluorescence Live-Cell Imaging for Glucosome Dynamics in Living Human Cells.
    • Authors: An S, Parajuli P, Kennedy EL, Kyoung M
    • Issue date: 2022
    • A Mathematical Model for Enzyme Clustering in Glucose Metabolism.
    • Authors: Jeon M, Kang HW, An S
    • Issue date: 2018 Feb 9
    • Phase-separated condensates of metabolic complexes in living cells: Purinosome and glucosome.
    • Authors: An S, Jeon M, Kennedy EL, Kyoung M
    • Issue date: 2019
    • Epidermal growth factor induces c-fos and c-jun mRNA via Raf-1/MEK1/ERK-dependent and -independent pathways in bovine luteal cells.
    • Authors: Chen DB, Davis JS
    • Issue date: 2003 Feb 28
    • Identification of a multienzyme complex for glucose metabolism in living cells.
    • Authors: Kohnhorst CL, Kyoung M, Jeon M, Schmitt DL, Kennedy EL, Ramirez J, Bracey SM, Luu BT, Russell SJ, An S
    • Issue date: 2017 Jun 2
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.