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dc.contributor.authorFrance, Michael T
dc.contributor.authorFu, Li
dc.contributor.authorRutt, Lindsay
dc.contributor.authorYang, Hongqiu
dc.contributor.authorHumphrys, Michael S
dc.contributor.authorNarina, Shilpa
dc.contributor.authorGajer, Pawel M
dc.contributor.authorMa, Bing
dc.contributor.authorForney, Larry J
dc.contributor.authorRavel, Jacques
dc.date.accessioned2022-03-14T12:36:56Z
dc.date.available2022-03-14T12:36:56Z
dc.date.issued2022-03-01
dc.identifier.urihttp://hdl.handle.net/10713/18219
dc.description.abstractBackground: Vaginal bacterial communities dominated by Lactobacillus species are associated with a reduced risk of various adverse health outcomes. However, somewhat unexpectedly, many healthy women have microbiota that are not dominated by lactobacilli. To determine the factors that drive vaginal community composition we characterized the genetic composition and transcriptional activities of vaginal microbiota in healthy women. Results: We demonstrate that the abundance of a species is not always indicative of its transcriptional activity and that impending changes in community composition can be predicted from metatranscriptomic data. Functional comparisons highlight differences in the metabolic activities of these communities, notably in their degradation of host produced mucin but not glycogen. Degradation of mucin by communities not dominated by Lactobacillus may play a role in their association with adverse health outcomes. Finally, we show that the transcriptional activities of L. crispatus, L. iners, and Gardnerella vaginalis vary with the taxonomic composition of the communities in which they reside. Notably, L. iners and G. vaginalis both demonstrate lower expression of their cholesterol-dependent cytolysins when co-resident with Lactobacillus spp. and higher expression when co-resident with other facultative and obligate anaerobes. The pathogenic potential of these species may depend on the communities in which they reside and thus could be modulated by interventional strategies. Conclusions: Our results provide insight to the functional ecology of the vaginal microbiota, demonstrate the diagnostic potential of metatranscriptomic data, and reveal strategies for the management of these ecosystems. © 2022, The Author(s).en_US
dc.description.urihttps://doi.org/10.1186/s13059-022-02635-9en_US
dc.language.isoenen_US
dc.publisherSpring Natureen_US
dc.relation.ispartofGenome Biologyen_US
dc.rights© 2022. The Author(s).en_US
dc.subjectHost-microbe interactionsen_US
dc.subjectMetagenomeen_US
dc.subjectMetatranscriptomeen_US
dc.subjectMicrobial ecologyen_US
dc.subjectVaginal microbiomeen_US
dc.titleInsight into the ecology of vaginal bacteria through integrative analyses of metagenomic and metatranscriptomic data.en_US
dc.typeArticleen_US
dc.identifier.doi10.1186/s13059-022-02635-9
dc.identifier.pmid35232471
dc.source.journaltitleGenome biology
dc.source.volume23
dc.source.issue1
dc.source.beginpage66
dc.source.endpage
dc.source.countryUnited States
dc.source.countryEngland


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