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dc.contributor.authorDeming, Meagan E
dc.contributor.authorDong, Tracy Q
dc.contributor.authorAgrawal, Vaidehi
dc.contributor.authorMills, Margaret G
dc.contributor.authorHuang, Meei-Li W
dc.contributor.authorGreninger, Alexander L
dc.contributor.authorJerome, Keith R
dc.contributor.authorWener, Mark H
dc.contributor.authorPaasche-Orlow, Michael K
dc.contributor.authorKissinger, Patricia
dc.contributor.authorLuk, Alfred
dc.contributor.authorHoffman, Risa M
dc.contributor.authorStewart, Jenell
dc.contributor.authorKottkamp, Angelica C
dc.contributor.authorBershteyn, Anna
dc.contributor.authorChu, Helen Y
dc.contributor.authorStankiewicz Karita, Helen C
dc.contributor.authorJohnston, Christine M
dc.contributor.authorWald, Anna
dc.contributor.authorBarnabas, Ruanne
dc.contributor.authorBrown, Elizabeth
dc.contributor.authorNeuzil, Kathleen
dc.date.accessioned2022-03-11T13:16:18Z
dc.date.available2022-03-11T13:16:18Z
dc.date.issued2022-02-12
dc.identifier.urihttp://hdl.handle.net/10713/18209
dc.description.abstractWhile detection of SARS-CoV-2 by diagnostic RT-PCR is highly sensitive for viral RNA, the nucleic acid amplification of subgenomic RNAs (sgRNA) that are the product of viral replication may more accurately identify replication. We characterized the diagnostic RT-PCR and sgRNA detection from nasal swabs collected daily by participants in post exposure prophylaxis or treatment studies for SARS-CoV-2. Among 1932 RT-PCR-positive swabs with sgRNA tests, 40% (767) had detectable sgRNA. Above a diagnostic PCR viral load threshold of 5.1 log10 copies/mL, 96% of samples had detectable sgRNA with viral loads that followed a linear trend. The trajectories of diagnostic and sgRNA viral loads differed, with 80% peaking on the same day but duration of sgRNA detection being shorter (8 versus 14 days). With a large sample of daily swabs we provide comparative sgRNA kinetics and a diagnostic PCR threshold that correlates with replicating virus independent of symptoms or duration of illness.en_US
dc.description.urihttps://doi.org/10.1093/infdis/jiac048en_US
dc.description.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/35150571/en_US
dc.language.isoenen_US
dc.publisherOxford University Pressen_US
dc.relation.ispartofJournal of Infectious Diseasesen_US
dc.rights© The Author(s) 2022. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.en_US
dc.subjectCOVID-19en_US
dc.subjectSARS-CoV-2en_US
dc.subjectdetectionen_US
dc.subjectkineticsen_US
dc.subjectsubgenomic RNAen_US
dc.subjectviral loaden_US
dc.titleDetection and kinetics of subgenomic SARS-CoV-2 RNA viral load in longitudinal diagnostic RNA positive samples.en_US
dc.typeArticleen_US
dc.identifier.doi10.1093/infdis/jiac048
dc.identifier.pmid35150571
dc.source.journaltitleThe Journal of infectious diseases
dc.source.countryUnited States


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