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dc.contributor.authorGerster, Tim
dc.contributor.authorWröbel, Michelle
dc.contributor.authorHofstaedter, Casey E
dc.contributor.authorSchwudke, Dominik
dc.contributor.authorErnst, Robert K
dc.contributor.authorRanf, Stefanie
dc.contributor.authorGisch, Nicolas
dc.date.accessioned2022-03-01T13:54:48Z
dc.date.available2022-03-01T13:54:48Z
dc.date.issued2022-02-11
dc.identifier.urihttp://hdl.handle.net/10713/18127
dc.description.abstractPseudomonas species infect a variety of organisms, including mammals and plants. Mammalian pathogens of the Pseudomonas family modify their lipid A during host entry to evade immune responses and to create an effective barrier against different environments, for example by removal of primary acyl chains, addition of phosphoethanolamine (P-EtN) to primary phosphates, and hydroxylation of secondary acyl chains. For Pseudomonas syringae pv. phaseolicola (Pph) 1448A, an economically important pathogen of beans, we observed similar lipid A modifications by mass spectrometric analysis. Therefore, we investigated predicted proteomes of various plant-associated Pseudomonas spp. for putative lipid A-modifying proteins using the well-studied mammalian pathogen Pseudomonas aeruginosa as a reference. We generated isogenic mutant strains of candidate genes and analyzed their lipid A. We show that the function of PagL, LpxO, and EptA is generally conserved in Pph 1448A. PagL-mediated de-acylation occurs at the distal glucosamine, whereas LpxO hydroxylates the secondary acyl chain on the distal glucosamine. The addition of P-EtN catalyzed by EptA occurs at both phosphates of lipid A. Our study characterizes lipid A modifications in vitro and provides a useful set of mutant strains relevant for further functional studies on lipid A modifications in Pph 1448A.en_US
dc.description.urihttps://doi.org/10.3390/ijms23041996en_US
dc.language.isoenen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofInternational Journal of Molecular Sciencesen_US
dc.subjectPseudomonasen_US
dc.subjectlipid Aen_US
dc.subjectlipopolysaccharideen_US
dc.subjectlipopolysaccharide remodelingen_US
dc.subjectmass spectrometryen_US
dc.titleRemodeling of Lipid A in pv. In Vitro.en_US
dc.typeArticleen_US
dc.identifier.doi10.3390/ijms23041996
dc.identifier.pmid35216122
dc.source.journaltitleInternational journal of molecular sciences
dc.source.volume23
dc.source.issue4
dc.source.countrySwitzerland


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