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    An Antigenically Diverse, Representative Panel of Envelope Glycoproteins for Hepatitis C Virus Vaccine Development.

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    Author
    Salas, Jordan H
    Urbanowicz, Richard A
    Guest, Johnathan D
    Frumento, Nicole
    Figueroa, Alexis
    Clark, Kaitlyn E
    Keck, Zhenyong
    Cowton, Vanessa M
    Cole, Sarah J
    Patel, Arvind H
    Fuerst, Thomas R
    Drummer, Heidi E
    Major, Marian
    Tarr, Alexander W
    Ball, Jonathan K
    Law, Mansun
    Pierce, Brian G
    Foung, Steven K H
    Bailey, Justin R
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    Date
    2021-10-13
    Journal
    Gastroenterology
    Publisher
    Elsevier
    Type
    Article
    
    Metadata
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    See at
    https://doi.org/10.1053/j.gastro.2021.10.005
    Abstract
    Background & aims: Development of a prophylactic hepatitis C virus (HCV) vaccine will require accurate and reproducible measurement of neutralizing breadth of vaccine-induced antibodies. Currently available HCV panels may not adequately represent the genetic and antigenic diversity of circulating HCV strains, and the lack of standardization of these panels makes it difficult to compare neutralization results obtained in different studies. Here, we describe the selection and validation of a genetically and antigenically diverse reference panel of 15 HCV pseudoparticles (HCVpps) for neutralization assays. Methods: We chose 75 envelope (E1E2) clones to maximize representation of natural polymorphisms observed in circulating HCV isolates, and 65 of these clones generated functional HCVpps. Neutralization sensitivity of these HCVpps varied widely. HCVpps clustered into 15 distinct groups based on patterns of relative sensitivity to 7 broadly neutralizing monoclonal antibodies. We used these data to select a final panel of 15 antigenically representative HCVpps. Results: Both the 65 and 15 HCVpp panels span 4 tiers of neutralization sensitivity, and neutralizing breadth measurements for 7 broadly neutralizing monoclonal antibodies were nearly equivalent using either panel. Differences in neutralization sensitivity between HCVpps were independent of genetic distances between E1E2 clones. Conclusions: Neutralizing breadth of HCV antibodies should be defined using viruses spanning multiple tiers of neutralization sensitivity rather than panels selected solely for genetic diversity. We propose that this multitier reference panel could be adopted as a standard for the measurement of neutralizing antibody potency and breadth, facilitating meaningful comparisons of neutralization results from vaccine studies in different laboratories.
    Rights/Terms
    Copyright © 2022 AGA Institute. All rights reserved.
    Keyword
    Broadly Neutralizing Antibodies
    Hepatitis C Virus
    Neutralizing Breadth
    Vaccine
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/17949
    ae974a485f413a2113503eed53cd6c53
    10.1053/j.gastro.2021.10.005
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