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dc.contributor.authorSong, Jin
dc.contributor.authorVanbuskirk, Julia A.
dc.contributor.authorMerbs, Shannath L.
dc.date.accessioned2022-01-31T13:37:39Z
dc.date.available2022-01-31T13:37:39Z
dc.date.issued2022-02-01
dc.identifier.urihttp://hdl.handle.net/10713/17811
dc.description.abstractOne important role of epigenetic regulation is controlling gene expression in development and homeostasis. However, little is known about epigenetics’ role in regulating opsin expression. Cell cultures (HEK 293, Y79, and WERI) producing different levels of opsins were treated with 5-aza-2’-deoxycytidine (5-Aza-dc) and/or sodium butyrate (SB) or suberoylanilide hydroxamic acid (SAHA) for 72 h. Global DNA methylation, site-specific methylation, and expressions of opsins were measured by LUMA assay, bisulfite pyrosequencing, and qPCR, respectively. Mouse retinal explants from wild-type P0/P1 pups were ex vivo cultured with/without 5-Aza-dc or SAHA for 6 days. The morphology of explants, DNA methylation, and expressions of opsins was examined. The drugs induced global DNA hypomethylation or increased histone acetylation in cells, including DNA hypomethylation of rhodopsin (RHO) and L-opsin (OPN1LW) and a concomitant increase in their expression. Further upregulation of RHO and/or OPN1LW in HEK 293 or WERI cells was observed with 5-Aza-dc and either SB or SAHA combination treatment. Mouse retinal explants developed normally but had drug-dependent differential DNA methylation and expression patterns of opsins. DNA methylation and histone acetylation directly regulate opsin expression both in vitro and ex vivo. The ability to manipulate opsin expression using epigenetic modifiers enables further study into the role of epigenetics in eye development and disease. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.en_US
dc.description.sponsorshipResearch to Prevent Blindnessen_US
dc.description.urihttps://doi.org/10.3390/ijms23031408en_US
dc.language.isoenen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofInternational Journal of Molecular Sciencesen_US
dc.subjectDNA methylationen_US
dc.subjectHistone acetylationen_US
dc.subjectOpsinen_US
dc.subjectRetinaen_US
dc.titleRegulation of Opsin Gene Expression by DNA Methylation and Histone Acetylationen_US
dc.typeArticleen_US
dc.identifier.doi10.3390/ijms23031408
dc.source.journaltitleInternational Journal of Molecular Sciences
dc.source.volume23
dc.source.issue3


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