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    Distribution of serotypes and antibiotic resistance of invasive Pseudomonas aeruginosa in a multi-country collection.

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    Author
    Nasrin, Shamima
    Hegerle, Nicolas
    Sen, Shaichi
    Nkeze, Joseph
    Sen, Sunil
    Permala-Booth, Jasnehta
    Choi, Myeongjin
    Sinclair, James
    Tapia, Milagritos D
    Johnson, J Kristie
    Sow, Samba O
    Thaden, Joshua T
    Fowler, Vance G
    Krogfelt, Karen A
    Brauner, Annelie
    Protonotariou, Efthymia
    Christaki, Eirini
    Shindo, Yuichiro
    Kwa, Andrea L
    Shakoor, Sadia
    Singh-Moodley, Ashika
    Perovic, Olga
    Jacobs, Jan
    Lunguya, Octavie
    Simon, Raphael
    Cross, Alan S
    Tennant, Sharon M
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    Date
    2022-01-06
    Journal
    BMC Microbiology
    Publisher
    Springer Nature
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1186/s12866-021-02427-4
    Abstract
    Background: Pseudomonas aeruginosa is an opportunistic pathogen that causes a wide range of acute and chronic infections and is frequently associated with healthcare-associated infections. Because of its ability to rapidly acquire resistance to antibiotics, P. aeruginosa infections are difficult to treat. Alternative strategies, such as a vaccine, are needed to prevent infections. We collected a total of 413 P. aeruginosa isolates from the blood and cerebrospinal fluid of patients from 10 countries located on 4 continents during 2005–2017 and characterized these isolates to inform vaccine development efforts. We determined the diversity and distribution of O antigen and flagellin types and antibiotic susceptibility of the invasive P. aeruginosa. We used an antibody-based agglutination assay and PCR for O antigen typing and PCR for flagellin typing. We determined antibiotic susceptibility using the Kirby-Bauer disk diffusion method. Results: Of the 413 isolates, 314 (95%) were typed by an antibody-based agglutination assay or PCR (n = 99). Among the 20 serotypes of P. aeruginosa, the most common serotypes were O1, O2, O3, O4, O5, O6, O8, O9, O10 and O11; a vaccine that targets these 10 serotypes would confer protection against more than 80% of invasive P. aeruginosa infections. The most common flagellin type among 386 isolates was FlaB (41%). Resistance to aztreonam (56%) was most common, followed by levofloxacin (42%). We also found that 22% of strains were non-susceptible to meropenem and piperacillin-tazobactam. Ninety-nine (27%) of our collected isolates were resistant to multiple antibiotics. Isolates with FlaA2 flagellin were more commonly multidrug resistant (p = 0.04). Conclusions: Vaccines targeting common O antigens and two flagellin antigens, FlaB and FlaA2, would offer an excellent strategy to prevent P. aeruginosa invasive infections. © 2022, The Author(s).
    Rights/Terms
    © 2022. The Author(s).
    Keyword
    Flagellin
    Multidrug resistance
    Pseudomonas
    Serotype
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/17506
    ae974a485f413a2113503eed53cd6c53
    10.1186/s12866-021-02427-4
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