Distribution of serotypes and antibiotic resistance of invasive Pseudomonas aeruginosa in a multi-country collection.
Tapia, Milagritos D
Johnson, J Kristie
Sow, Samba O
Thaden, Joshua T
Fowler, Vance G
Krogfelt, Karen A
Kwa, Andrea L
Cross, Alan S
Tennant, Sharon M
MetadataShow full item record
AbstractBackground: Pseudomonas aeruginosa is an opportunistic pathogen that causes a wide range of acute and chronic infections and is frequently associated with healthcare-associated infections. Because of its ability to rapidly acquire resistance to antibiotics, P. aeruginosa infections are difficult to treat. Alternative strategies, such as a vaccine, are needed to prevent infections. We collected a total of 413 P. aeruginosa isolates from the blood and cerebrospinal fluid of patients from 10 countries located on 4 continents during 2005–2017 and characterized these isolates to inform vaccine development efforts. We determined the diversity and distribution of O antigen and flagellin types and antibiotic susceptibility of the invasive P. aeruginosa. We used an antibody-based agglutination assay and PCR for O antigen typing and PCR for flagellin typing. We determined antibiotic susceptibility using the Kirby-Bauer disk diffusion method. Results: Of the 413 isolates, 314 (95%) were typed by an antibody-based agglutination assay or PCR (n = 99). Among the 20 serotypes of P. aeruginosa, the most common serotypes were O1, O2, O3, O4, O5, O6, O8, O9, O10 and O11; a vaccine that targets these 10 serotypes would confer protection against more than 80% of invasive P. aeruginosa infections. The most common flagellin type among 386 isolates was FlaB (41%). Resistance to aztreonam (56%) was most common, followed by levofloxacin (42%). We also found that 22% of strains were non-susceptible to meropenem and piperacillin-tazobactam. Ninety-nine (27%) of our collected isolates were resistant to multiple antibiotics. Isolates with FlaA2 flagellin were more commonly multidrug resistant (p = 0.04). Conclusions: Vaccines targeting common O antigens and two flagellin antigens, FlaB and FlaA2, would offer an excellent strategy to prevent P. aeruginosa invasive infections. © 2022, The Author(s).
Rights/Terms© 2022. The Author(s).
Identifier to cite or link to this itemhttp://hdl.handle.net/10713/17506
- Association between Pseudomonas aeruginosa O-antigen serotypes, resistance profiles and high-risk clones: results from a Spanish nationwide survey.
- Authors: Del Barrio-Tofiño E, Sánchez-Diener I, Zamorano L, Cortes-Lara S, López-Causapé C, Cabot G, Bou G, Martínez-Martínez L, Oliver A, GEMARA-SEIMC/REIPI.
- Issue date: 2019 Nov 1
- Antimicrobial resistance and genomic rep-PCR fingerprints of Pseudomonas aeruginosa strains from animals on the background of the global population structure.
- Authors: Serrano I, Oliveira M, Santos JP, Bilocq F, Leitão A, Tavares L, Pirnay JP, De Vos D
- Issue date: 2017 Feb 21
- Serotype, antimicrobial susceptibility and clone distribution of Pseudomonas aeruginosa in a university hospital.
- Authors: Müller-Premru M, Gubina M
- Issue date: 2000 Jan
- Surveillance of Pseudomonas aeruginosa in intensive care units: clusters of nosocomial cross-infection and encounter of a multiple-antibiotic resistant strain.
- Authors: Traub WH, Scheidhauer R, Leonhard B, Bauer D
- Issue date: 1998 Jul-Aug
- The peculiarities of Pseudomonas aeruginosa resistance to antibiotics and prevalence of serogroups.
- Authors: Gailiene G, Pavilonis A, Kareiviene V
- Issue date: 2007