• Login
    View Item 
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Inhibition of endogenous ouabain by atrial natriuretic peptide is a guanylyl cyclase independent effect.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Author
    Tegin, Gulay
    Gao, Yonglin
    Hamlyn, John M
    Clark, Barbara J
    El-Mallakh, Rif S
    Date
    2021-11-18
    Journal
    PLoS ONE
    Publisher
    Public Library of Science
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1371/journal.pone.0260131
    Abstract
    BACKGROUND: Endogenous ouabain (EO) and atrial natriuretic peptide (ANP) are important in regulation of sodium and fluid balance. There is indirect evidence that ANP may be involved in the regulation of endogenous cardenolides. METHODS: H295R are human adrenocortical cells known to release EO. Cells were treated with ANP at physiologic concentrations or vehicle (0.1% DMSO), with or without guanylyl cyclase inhibitor 1,2,4 oxadiazolo[4,3-a]quinoxalin-1-one (ODQ). Cyclic guanosine monophosphate (cGMP), the intracellular second messenger of ANP, was measured by a chemiluminescent immunoassay and EO was measured by radioimmunoassay of C18 extracted samples. RESULTS: EO secretion is inhibited by ANP treatment, with the most prolonged inhibition (90 min vs ≤ 60 min) occurring at physiologic ANP concentrations (50 pg/mL). Inhibition of guanylyl cyclase with ODQ, also reduces EO secretion. The inhibitory effects on EO release in response to cotreatment with ANP and ODQ appeared to be additive. CONCLUSIONS: ANP inhibits basal EO secretion, and it is unlikely that this is mediated through ANP-A or ANP-B receptors (the most common natriuretic peptide receptors) or their cGMP second messenger; the underlying mechanisms involved are not revealed in the current studies. The role of ANP in the control of EO synthesis and secretion in vivo requires further investigation.
    Keyword
    endogenous ouabain
    Atrial Natriuretic Factor--physiology
    Ouabain
    Cardenolides
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/17259
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pone.0260131
    Scopus Count
    Collections
    UMB Open Access Articles

    entitlement

    Related articles

    • Expression of guanylyl cyclase-A/atrial natriuretic peptide receptor blocks the activation of protein kinase C in vascular smooth muscle cells. Role of cGMP and cGMP-dependent protein kinase.
    • Authors: Kumar R, Cartledge WA, Lincoln TM, Pandey KN
    • Issue date: 1997 Jan
    • The H295R human adrenocortical cell line contains functional atrial natriuretic peptide receptors that inhibit aldosterone biosynthesis.
    • Authors: Bodart V, Rainey WE, Fournier A, Ong H, De Léan A
    • Issue date: 1996 Apr 19
    • Evaluating atrial natriuretic peptide-induced cGMP production by particulate guanylyl cyclase stimulation in vitro and in vivo.
    • Authors: Hamet P, Tremblay J
    • Issue date: 1991
    • Relaxation and modulation of cyclic AMP production in response to atrial natriuretic peptides in guinea pig tracheal smooth muscle.
    • Authors: Devillier P, Corompt E, Bréant D, Caron F, Bessard G
    • Issue date: 2001 Nov 2
    • Inhibition by HS-142-1, a novel nonpeptide atrial natriuretic peptide antagonist of microbial origin, of atrial natriuretic peptide-induced relaxation of isolated rabbit aorta through the blockade of guanylyl cyclase-linked receptors.
    • Authors: Imura R, Sano T, Goto J, Yamada K, Matsuda Y
    • Issue date: 1992 Dec
    DSpace software (copyright © 2002 - 2022)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.