• Login
    View Item 
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Evaluation of mouse urinary bladder smooth muscle for diurnal differences in contractile properties

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Author
    White, Rachel S
    Zemen, Betsir G
    Khan, Zulqarnain
    Montgomery, Jenna R
    Herrera, Gerald M
    Meredith, Andrea L
    Date
    2015-01-09
    Journal
    Frontiers in Pharmacology
    Publisher
    Frontiers Media S.A.
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.3389/fphar.2014.00293
    Abstract
    Most physiological systems show daily variations in functional output, entrained to the day–night cycle. Humans exhibit a daily rhythm in urinary voiding (micturition), and disruption of this rhythm (nocturia) has significant clinical impact. However, the underlying mechanisms are not well-understood. Recently, a circadian rhythm in micturition was demonstrated in rodents, correlated with functional changes in urodynamics, providing the opportunity to address this issue in an animal model. Smooth muscle cells from mouse bladder have been proposed to express a functional and autonomous circadian clock at the molecular level. In this study, we addressed whether a semi-intact preparation of mouse urinary bladder smooth muscle (UBSM) exhibited measurable differences in contractility between day and night. UBSM tissue strips were harvested at four time points over the diurnal cycle, and spontaneous (phasic) and nerve-evoked contractions were assessed using isometric tension recordings. During the active period (ZT12-24) when micturition frequency is higher in rodents, UBSM strips had no significant differences in maximal- (high K+) or nerve-evoked contractions compared to strips harvested from the resting period (ZT0-12). However, a diurnal rhythm in phasic contraction was observed, with higher amplitudes at ZT10. Consistent with the enhanced phasic amplitudes, expression of the BK K+ channel, a key suppressor of UBSM excitability, was lower at ZT8. Higher expression of BK at ZT20 was correlated with an enhanced effect of the BK antagonist paxilline (PAX) on phasic amplitude, but PAX had no significant time-of-day dependent effect on phasic frequency or nerve-evoked contractions. Overall, these results identify a diurnal difference for one contractile parameter of bladder muscle. Taken together, the results suggest that autonomous clocks in UBSM make only a limited contribution to the integrated control of diurnal micturition patterns. Copyright © 2015 White, Zemen, Khan, Montgomery, Herrera and Meredith.
    Keyword
    BK channel
    Kcnma1
    UBSM
    circadian rhythm
    isometric tension
    lower urinary tract
    peripheral rhythm
    urodynamics
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/17254
    ae974a485f413a2113503eed53cd6c53
    10.3389/fphar.2014.00293
    Scopus Count
    Collections
    UMB Open Access Articles

    entitlement

    Related articles

    • Beta1-subunit of the Ca2+-activated K+ channel regulates contractile activity of mouse urinary bladder smooth muscle.
    • Authors: Petkov GV, Bonev AD, Heppner TJ, Brenner R, Aldrich RW, Nelson MT
    • Issue date: 2001 Dec 1
    • Constitutive PKA activity is essential for maintaining the excitability and contractility in guinea pig urinary bladder smooth muscle: role of the BK channel.
    • Authors: Xin W, Li N, Cheng Q, Fernandes VS, Petkov GV
    • Issue date: 2014 Dec 15
    • Beta-adrenergic relaxation of mouse urinary bladder smooth muscle in the absence of large-conductance Ca2+-activated K+ channel.
    • Authors: Brown SM, Bentcheva-Petkova LM, Liu L, Hristov KL, Chen M, Kellett WF, Meredith AL, Aldrich RW, Nelson MT, Petkov GV
    • Issue date: 2008 Oct
    • Urodynamic properties and neurotransmitter dependence of urinary bladder contractility in the BK channel deletion model of overactive bladder.
    • Authors: Thorneloe KS, Meredith AL, Knorn AM, Aldrich RW, Nelson MT
    • Issue date: 2005 Sep
    • Age-dependent decrease in TRPM4 channel expression but not trafficking alters urinary bladder smooth muscle contractility.
    • Authors: Maxwell SE, Leo MD, Malysz J, Petkov GV
    • Issue date: 2021 Feb
    DSpace software (copyright © 2002 - 2022)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.