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    Recent progress and new challenges in modeling of human pluripotent stem cell-derived blood-brain barrier

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    Author
    Yan, Li
    Moriarty, Rebecca A
    Stroka, Kimberly M
    Date
    2021-11-02
    Journal
    Theranostics
    Publisher
    Ivyspring International Publisher
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.7150/thno.63195
    Abstract
    The blood-brain barrier (BBB) is a semipermeable unit that serves to vascularize the central nervous system (CNS) while tightly regulating the movement of molecules, ions, and cells between the blood and the brain. The BBB precisely controls brain homeostasis and protects the neural tissue from toxins and pathogens. The BBB is coordinated by a tight monolayer of brain microvascular endothelial cells, which is subsequently supported by mural cells, astrocytes, and surrounding neuronal cells that regulate the barrier function with a series of specialized properties. Dysfunction of barrier properties is an important pathological feature in the progression of various neurological diseases. In vitro BBB models recapitulating the physiological and diseased states are important tools to understand the pathological mechanism and to serve as a platform to screen potential drugs. Recent advances in this field have stemmed from the use of pluripotent stem cells (PSCs). Various cell types of the BBB such as brain microvascular endothelial cells (BMECs), pericytes, and astrocytes have been derived from PSCs and synergistically incorporated to model the complex BBB structure in vitro. In this review, we summarize the most recent protocols and techniques for the differentiation of major cell types of the BBB. We also discuss the progress of BBB modeling by using PSC-derived cells and perspectives on how to reproduce more natural BBBs in vitro.
    Rights/Terms
    © The author(s).
    Keyword
    blood-brain barrier
    brain microvascular endothelial cells
    disease modeling.
    neurovascular unit
    pluripotent stem cells
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/17215
    ae974a485f413a2113503eed53cd6c53
    10.7150/thno.63195
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