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dc.contributor.authorUdagawa, Tomokatsu
dc.contributor.authorAtkinson, Patrick J
dc.contributor.authorMilon, Beatrice
dc.contributor.authorAbitbol, Julia M
dc.contributor.authorSong, Yang
dc.contributor.authorSperber, Michal
dc.contributor.authorHuarcaya Najarro, Elvis
dc.contributor.authorScheibinger, Mirko
dc.contributor.authorElkon, Ran
dc.contributor.authorHertzano, Ronna
dc.contributor.authorCheng, Alan G
dc.date.accessioned2021-11-18T19:14:51Z
dc.date.available2021-11-18T19:14:51Z
dc.date.issued2021-11-10
dc.identifier.urihttp://hdl.handle.net/10713/17164
dc.description.abstractCochlear supporting cells (SCs) are glia-like cells critical for hearing function. In the neonatal cochlea, the greater epithelial ridge (GER) is a mitotically quiescent and transient organ, which has been shown to nonmitotically regenerate SCs. Here, we ablated Lgr5+ SCs using Lgr5-DTR mice and found mitotic regeneration of SCs by GER cells in vivo. With lineage tracing, we show that the GER houses progenitor cells that robustly divide and migrate into the organ of Corti to replenish ablated SCs. Regenerated SCs display coordinated calcium transients, markers of the SC subtype inner phalangeal cells, and survive in the mature cochlea. Via RiboTag, RNA-sequencing, and gene clustering algorithms, we reveal 11 distinct gene clusters comprising markers of the quiescent and damaged GER, and damage-responsive genes driving cell migration and mitotic regeneration. Together, our study characterizes GER cells as mitotic progenitors with regenerative potential and unveils their quiescent and damaged translatomes.en_US
dc.description.urihttps://doi.org/10.1371/journal.pbio.3001445en_US
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPLoS Biologyen_US
dc.subjectcochlear supporting cells (SCs)en_US
dc.subjectmitotic regenerationen_US
dc.subjecttranslatomic analysisen_US
dc.titleLineage-tracing and translatomic analysis of damage-inducible mitotic cochlear progenitors identifies candidate genes regulating regeneration.en_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pbio.3001445
dc.identifier.pmid34758021
dc.source.journaltitlePLoS biology
dc.source.volume19
dc.source.issue11
dc.source.beginpagee3001445
dc.source.endpage
dc.source.countryUnited States


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