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dc.contributor.authorXu, Rui
dc.contributor.authorDu, Shaojun
dc.date.accessioned2021-11-16T13:00:55Z
dc.date.available2021-11-16T13:00:55Z
dc.date.issued2021-10-26
dc.identifier.urihttp://hdl.handle.net/10713/17123
dc.description.abstractLifeact-GFP is a frequently used molecular probe to study F-actin structure and dynamic assembly in living cells. In this study, we generated transgenic zebrafish models expressing Lifeact-GFP specifically in cardiac muscles to investigate the effect of Lifeact-GFP on heart development and its application to study cardiomyopathy. The data showed that transgenic zebrafish with low to moderate levels of Lifeact-GFP expression could be used as a good model to study contractile dynamics of actin filaments in cardiac muscles in vivo. Using this model, we demonstrated that loss of Smyd1b, a lysine methyltransferase, disrupted F-actin filament organization in cardiomyocytes of zebrafish embryos. Our studies, however, also demonstrated that strong Lifeact-GFP expression in cardiomyocytes was detrimental to actin filament organization in cardiomyocytes that led to pericardial edema and early embryonic lethality of zebrafish embryos. Collectively, these data suggest that although Lifeact-GFP is a good probe for visualizing F-actin dynamics, transgenic models need to be carefully evaluated to avoid artifacts induced by Lifeact-GFP overexpression.en_US
dc.description.sponsorshipNational Institute of Arthritis and Musculoskeletal and Skin Diseasesen_US
dc.description.urihttps://doi.org/10.3389/fcell.2021.746818en_US
dc.language.isoenen_US
dc.publisherFrontiers Media SAen_US
dc.relation.ispartofFrontiers in Cell and Developmental Biologyen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectF-actin filamenten_US
dc.subjectLifeact-GFPen_US
dc.subjectSmyd1en_US
dc.subjectcardiomyocyteen_US
dc.subjectsarcomereen_US
dc.titleOverexpression of Lifeact-GFP Disrupts F-Actin Organization in Cardiomyocytes and Impairs Cardiac Functionen_US
dc.typeArticleen_US
dc.identifier.doi10.3389/fcell.2021.746818
dc.source.volume9


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