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    STK38 is a PPARγ-interacting protein promoting adipogenesis

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    Author
    Qian, Kun
    Yu, Daozhan
    Wang, Weiming
    Jiang, Mengqi
    Yang, Rongze
    Brown, Robert
    Gong, Da-Wei
    Date
    2021-10-20
    Journal
    Adipocyte
    Publisher
    Taylor and Francis Inc.
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1080/21623945.2021.1980257
    Abstract
    Peroxisome proliferator-activated receptor-γ (PPARγ) is the master regulator of adipogenesis, but knowledge about how PPARγ is regulated at the protein level is very limited. We aimed to identify PPARγ-interacting proteins which modulate PPARγ’s protein levels and transactivating activities in human adipocytes. We expressed Flag-tagged PPARγ in human preadipocytes as bait to capture PPARγ-associated proteins, followed by mass spectroscopy and proteomics analysis, which identified serine/threonine kinase 38 (STK38) as a major PPARγ-associated protein. Protein pulldown studies confirmed this protein–protein interaction in transfected cells, and reporter assays demonstrated that STK38 enhanced PPARγ’s transactivating activities without requiring STK38’s kinase activity. In cell-based assays, STK38 increased PPARγ protein stability, extending PPARγ’s half-life from ~1.08 to 1.95 h. Notably, in human preadipocytes, the overexpression of STK38 enhanced adipogenesis, whereas knockdown impaired the process in a PPARγ-dependent manner. Thus, we discovered that STK38 is a novel PPARγ-cofactor promoting adipogenesis, likely through stabilization of PPARγ. © 2021 The Author(s).
    Keyword
    PPARγ
    STK38
    cofactors and adipocytes
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/17028
    ae974a485f413a2113503eed53cd6c53
    10.1080/21623945.2021.1980257
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