• Login
    View Item 
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    A novel procedure in combination of genomic sequencing, flow cytometry and routine culturing for confirmation of beer spoilage caused by Pediococcus damnosus in viable but nonculturable state

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Publisher version
    View Source
    Access full-text PDFOpen Access
    View Source
    Check access options
    Check access options
    Author
    Xu, Zhenbo
    Wang, Kan
    Liu, Ziqi
    Soteyome, Thanapop
    Deng, Yang
    Chen, Ling
    Seneviratne, Gamini
    Hong, Wei
    Liu, Junyan
    Harro, Janette M.
    Kjellerup, Birthe V.
    Show allShow less

    Date
    2021-10-14
    Journal
    LWT
    Publisher
    Academic Press Inc.
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1016/j.lwt.2021.112623
    Abstract
    Spoilage bacteria had been shown to form viable but nonculturable (VBNC) state maintaining food spoilage capability. In this study, a novel procedure was used to confirm a beer spoilage case caused by a Pediococcus damnosus strain in the VBNC state. Firstly, flow cytometry, routine culturing and PMA-PCR methods were used to identify approximately 103 cells/ml VBNC cells in the spoiled beer sample based on the difference between CFU and viable cell numbers. Secondly, genomic sequencing showed all acquired scaffolds were identical to the genome of P. damnosus with no existence of other species or isolates. In addition, VBNC cells were obtained in both simulation conditions, including beer low temperature storage and subculturing. MRS agar supplemented with catalase was found to resuscitate VBNC cells. Normal, VBNC and resuscitated cells showed similar level of beer spoilage capability. As concluded, a novel procedure, in combination of genomic sequencing, flow cytometry and routine culturing was used to confirm VBNC cells in spoiled beer sample, providing direct evidence on the beer spoilage caused by VBNC P. damnosus cells, and will aid in further study on VBNC state in food industry so that more evidence on food safety problem caused by VBNC microbes will be shown. © 2021 The Authors
    Sponsors
    Higher Education Discipline Innovation Project
    Keyword
    Beer spoilage
    Flow cytometry
    Pediococcus damnosus
    Resuscitation
    Viable but nonculturable state (VBNC)
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/17009
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.lwt.2021.112623
    Scopus Count
    Collections
    UMB Open Access Articles

    entitlement

     
    DSpace software (copyright © 2002 - 2022)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.