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dc.contributor.authorBakken, Trygve E
dc.contributor.authorJorstad, Nikolas L
dc.contributor.authorHu, Qiwen
dc.contributor.authorLake, Blue B
dc.contributor.authorTian, Wei
dc.contributor.authorKalmbach, Brian E
dc.contributor.authorCrow, Megan
dc.contributor.authorHodge, Rebecca D
dc.contributor.authorKrienen, Fenna M
dc.contributor.authorSorensen, Staci A
dc.contributor.authorEggermont, Jeroen
dc.contributor.authorYao, Zizhen
dc.contributor.authorAevermann, Brian D
dc.contributor.authorAldridge, Andrew I
dc.contributor.authorBartlett, Anna
dc.contributor.authorBertagnolli, Darren
dc.contributor.authorCasper, Tamara
dc.contributor.authorCastanon, Rosa G
dc.contributor.authorCrichton, Kirsten
dc.contributor.authorDaigle, Tanya L
dc.contributor.authorDalley, Rachel
dc.contributor.authorDee, Nick
dc.contributor.authorDembrow, Nikolai
dc.contributor.authorDiep, Dinh
dc.contributor.authorDing, Song-Lin
dc.contributor.authorDong, Weixiu
dc.contributor.authorFang, Rongxin
dc.contributor.authorFischer, Stephan
dc.contributor.authorGoldman, Melissa
dc.contributor.authorGoldy, Jeff
dc.contributor.authorGraybuck, Lucas T
dc.contributor.authorHerb, Brian R
dc.contributor.authorHou, Xiaomeng
dc.contributor.authorKancherla, Jayaram
dc.contributor.authorKroll, Matthew
dc.contributor.authorLathia, Kanan
dc.contributor.authorvan Lew, Baldur
dc.contributor.authorLi, Yang Eric
dc.contributor.authorLiu, Christine S
dc.contributor.authorLiu, Hanqing
dc.contributor.authorLucero, Jacinta D
dc.contributor.authorMahurkar, Anup
dc.contributor.authorMcMillen, Delissa
dc.contributor.authorMiller, Jeremy A
dc.contributor.authorMoussa, Marmar
dc.contributor.authorNery, Joseph R
dc.contributor.authorNicovich, Philip R
dc.contributor.authorNiu, Sheng-Yong
dc.contributor.authorOrvis, Joshua
dc.contributor.authorOsteen, Julia K
dc.contributor.authorOwen, Scott
dc.contributor.authorPalmer, Carter R
dc.contributor.authorPham, Thanh
dc.contributor.authorPlongthongkum, Nongluk
dc.contributor.authorPoirion, Olivier
dc.contributor.authorReed, Nora M
dc.contributor.authorRimorin, Christine
dc.contributor.authorRivkin, Angeline
dc.contributor.authorRomanow, William J
dc.contributor.authorSedeño-Cortés, Adriana E
dc.contributor.authorSiletti, Kimberly
dc.contributor.authorSomasundaram, Saroja
dc.contributor.authorSulc, Josef
dc.contributor.authorTieu, Michael
dc.contributor.authorTorkelson, Amy
dc.contributor.authorTung, Herman
dc.contributor.authorWang, Xinxin
dc.contributor.authorXie, Fangming
dc.contributor.authorYanny, Anna Marie
dc.contributor.authorZhang, Renee
dc.contributor.authorAment, Seth A
dc.contributor.authorBehrens, M Margarita
dc.contributor.authorBravo, Hector Corrada
dc.contributor.authorChun, Jerold
dc.contributor.authorDobin, Alexander
dc.contributor.authorGillis, Jesse
dc.contributor.authorHertzano, Ronna
dc.contributor.authorHof, Patrick R
dc.contributor.authorHöllt, Thomas
dc.contributor.authorHorwitz, Gregory D
dc.contributor.authorKeene, C Dirk
dc.contributor.authorKharchenko, Peter V
dc.contributor.authorKo, Andrew L
dc.contributor.authorLelieveldt, Boudewijn P
dc.contributor.authorLuo, Chongyuan
dc.contributor.authorMukamel, Eran A
dc.contributor.authorPinto-Duarte, António
dc.contributor.authorPreissl, Sebastian
dc.contributor.authorRegev, Aviv
dc.contributor.authorRen, Bing
dc.contributor.authorScheuermann, Richard H
dc.contributor.authorSmith, Kimberly
dc.contributor.authorSpain, William J
dc.contributor.authorWhite, Owen R
dc.contributor.authorKoch, Christof
dc.contributor.authorHawrylycz, Michael
dc.contributor.authorTasic, Bosiljka
dc.contributor.authorMacosko, Evan Z
dc.contributor.authorMcCarroll, Steven A
dc.contributor.authorTing, Jonathan T
dc.contributor.authorZeng, Hongkui
dc.contributor.authorZhang, Kun
dc.contributor.authorFeng, Guoping
dc.contributor.authorEcker, Joseph R
dc.contributor.authorLinnarsson, Sten
dc.contributor.authorLein, Ed S
dc.date.accessioned2021-10-19T19:21:17Z
dc.date.available2021-10-19T19:21:17Z
dc.date.issued2021-10-06
dc.identifier.urihttp://hdl.handle.net/10713/16883
dc.description.abstractThe primary motor cortex (M1) is essential for voluntary fine-motor control and is functionally conserved across mammals1. Here, using high-throughput transcriptomic and epigenomic profiling of more than 450,000 single nuclei in humans, marmoset monkeys and mice, we demonstrate a broadly conserved cellular makeup of this region, with similarities that mirror evolutionary distance and are consistent between the transcriptome and epigenome. The core conserved molecular identities of neuronal and non-neuronal cell types allow us to generate a cross-species consensus classification of cell types, and to infer conserved properties of cell types across species. Despite the overall conservation, however, many species-dependent specializations are apparent, including differences in cell-type proportions, gene expression, DNA methylation and chromatin state. Few cell-type marker genes are conserved across species, revealing a short list of candidate genes and regulatory mechanisms that are responsible for conserved features of homologous cell types, such as the GABAergic chandelier cells. This consensus transcriptomic classification allows us to use patch-seq (a combination of whole-cell patch-clamp recordings, RNA sequencing and morphological characterization) to identify corticospinal Betz cells from layer 5 in non-human primates and humans, and to characterize their highly specialized physiology and anatomy. These findings highlight the robust molecular underpinnings of cell-type diversity in M1 across mammals, and point to the genes and regulatory pathways responsible for the functional identity of cell types and their species-specific adaptations.en_US
dc.description.urihttps://www.nature.com/articles/s41586-021-03465-8en_US
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.relation.ispartofNatureen_US
dc.rights© 2021. The Author(s).en_US
dc.subjectcomparative cellular analysisen_US
dc.subjectepigenomic profilingen_US
dc.subjecttranscriptomic profilingen_US
dc.subject.meshMotor Cortexen_US
dc.subject.meshCallithrixen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.titleComparative cellular analysis of motor cortex in human, marmoset and mouseen_US
dc.typeArticleen_US
dc.identifier.doi10.1038/s41586-021-03465-8
dc.identifier.pmid34616062
dc.source.volume598
dc.source.issue7879
dc.source.beginpage111
dc.source.endpage119
dc.source.countryUnited States
dc.source.countryEngland


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