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    An adaptive optics module for deep tissue multiphoton imaging in vivo

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    Author
    Rodríguez, Cristina
    Chen, Anderson
    Rivera, José A
    Mohr, Manuel A
    Liang, Yajie
    Natan, Ryan G
    Sun, Wenzhi
    Milkie, Daniel E
    Bifano, Thomas G
    Chen, Xiaoke
    Ji, Na
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    Date
    2021-10-04
    Journal
    Nature Methods
    Publisher
    Springer Nature
    Type
    Article
    
    Metadata
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    See at
    https://doi.org/10.1038/s41592-021-01279-0
    https://www.biorxiv.org/content/10.1101/2020.11.25.397968v1
    Abstract
    Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at great depths in vivo.
    Rights/Terms
    © 2021. The Author(s), under exclusive licence to Springer Nature America, Inc.
    Keyword
    adaptive optics module
    deep tissue imaging
    multiphoton imaging
    Intravital Microscopy--methods
    Mice
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/16879
    ae974a485f413a2113503eed53cd6c53
    10.1038/s41592-021-01279-0
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