An adaptive optics module for deep tissue multiphoton imaging in vivo
Rivera, José A
Mohr, Manuel A
Natan, Ryan G
Milkie, Daniel E
Bifano, Thomas G
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AbstractUnderstanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at great depths in vivo.
Rights/Terms© 2021. The Author(s), under exclusive licence to Springer Nature America, Inc.
Keywordadaptive optics module
deep tissue imaging
Identifier to cite or link to this itemhttp://hdl.handle.net/10713/16879
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