An adaptive optics module for deep tissue multiphoton imaging in vivo
Author
Rodríguez, CristinaChen, Anderson
Rivera, José A
Mohr, Manuel A
Liang, Yajie
Natan, Ryan G
Sun, Wenzhi
Milkie, Daniel E
Bifano, Thomas G
Chen, Xiaoke
Ji, Na
Date
2021-10-04Journal
Nature MethodsPublisher
Springer NatureType
Article
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https://doi.org/10.1038/s41592-021-01279-0https://www.biorxiv.org/content/10.1101/2020.11.25.397968v1
Abstract
Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at great depths in vivo.Rights/Terms
© 2021. The Author(s), under exclusive licence to Springer Nature America, Inc.Keyword
adaptive optics moduledeep tissue imaging
multiphoton imaging
Intravital Microscopy--methods
Mice
Identifier to cite or link to this item
http://hdl.handle.net/10713/16879ae974a485f413a2113503eed53cd6c53
10.1038/s41592-021-01279-0
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