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    A genotyping assay to determine geographic origin and transmission potential of Plasmodium falciparum malaria cases.

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    Author
    Molina-Cruz, Alvaro
    Raytselis, Nadia
    Withers, Roxanne
    Dwivedi, Ankit
    Crompton, Peter D
    Traore, Boubacar
    Carpi, Giovanna
    Silva, Joana C
    Barillas-Mury, Carolina
    Date
    2021-09-30
    Journal
    Communications Biology
    Publisher
    Springer Nature
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1038/s42003-021-02667-0
    Abstract
    As countries work towards malaria elimination, it is important to monitor imported cases to prevent reestablishment of local transmission. The Plasmodium falciparum Pfs47 gene has strong geographic population structure, because only those parasites with Pfs47 haplotypes compatible with the mosquito vector species in a given continent are efficiently transmitted. Analysis of 4,971 world-wide Pfs47 sequences identified two SNPs (at 707 and 725 bp) as sufficient to establish the likely continent of origin of P. falciparum isolates. Pfs47 sequences from Africa, Asia, and the New World presented more that 99% frequency of distinct combinations of the SNPs 707 and 725 genotypes. Interestingly, Papua New Guinea Pfs47 sequences have the highest diversity in SNPs 707 and 725. Accurate and reproducible High-Resolution Melting (HRM) assays were developed to genotype Pfs47 SNPs 707 and 725 in laboratory and field samples, to assess the geographic origin and risk of local transmission of imported P. falciparum malaria cases.
    Rights/Terms
    © 2021. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.
    Keyword
    continent of origin
    Malaria
    Plasmodium falciparum--genetics
    Polymorphism, Single Nucleotide
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/16805
    ae974a485f413a2113503eed53cd6c53
    10.1038/s42003-021-02667-0
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