Characteristic Microbiomes Correlate with Polyphosphate Accumulation of Marine Sponges in South China Sea Areas
MetadataShow full item record
AbstractSome sponges have been shown to accumulate abundant phosphorus in the form of polyphosphate (polyP) granules even in waters where phosphorus is present at low concentrations. But the polyP accumulation occurring in sponges and their symbiotic bacteria have been little studied. The amounts of polyP exhibited significant differences in twelve sponges from marine environments with high or low dissolved inorganic phosphorus (DIP) concentrations which were quantified by spectral analysis, even though in the same sponge genus, e.g., Mycale sp. or Callyspongia sp. PolyP enrichment rates of sponges in oligotrophic environments were far higher than those in eutrophic environments. Massive polyP granules were observed under confocal microscopy in samples from very low DIP environments. The composition of sponge symbiotic microbes was analyzed by high-throughput sequencing and the corresponding polyphosphate kinase (ppk) genes were detected. Sequence analysis revealed that in the low DIP environment, those sponges with higher polyP content and enrichment rates had relatively higher abundances of cyanobacteria. Mantel tests and canonical correspondence analysis (CCA) examined that the polyP enrichment rate was most strongly correlated with the structure of microbial communities, including genera Synechococcus, Rhodopirellula, Blastopirellula, and Rubripirellula. About 50% of ppk genes obtained from the total DNA of sponge holobionts, had above 80% amino acid sequence similarities to those sequences from Synechococcus. In general, it suggested that sponges employed differentiated strategies towards the use of phosphorus in different nutrient environments and the symbiotic Synechococcus could play a key role in accumulating polyP.
DescriptionCorrection (https://doi.org/10.3390/microorganisms9091826): There is a mistake in a unit of DIP in Surrounding Sea Water provided in Table 1. The unit of DIP in Surrounding Sea Water listed in the original version of the article was “mM”. The correct version should be as follows: The correct unit of DIP in Surrounding Sea Water in Table 1 is “µM”.
Identifier to cite or link to this itemhttp://hdl.handle.net/10713/16752
- Phosphorus sequestration in the form of polyphosphate by microbial symbionts in marine sponges.
- Authors: Zhang F, Blasiak LC, Karolin JO, Powell RJ, Geddes CD, Hill RT
- Issue date: 2015 Apr 7
- Metagenomic Analysis of Genes Encoding Nutrient Cycling Pathways in the Microbiota of Deep-Sea and Shallow-Water Sponges.
- Authors: Li Z, Wang Y, Li J, Liu F, He L, He Y, Wang S
- Issue date: 2016 Dec
- Potential Interactions between Clade SUP05 Sulfur-Oxidizing Bacteria and Phages in Hydrothermal Vent Sponges.
- Authors: Zhou K, Zhang R, Sun J, Zhang W, Tian RM, Chen C, Kawagucci S, Xu Y
- Issue date: 2019 Nov 15
- Polyphosphate kinase of Lysinibacillus sphaericus and its effects on accumulation of polyphosphate and bacterial growth.
- Authors: Shi T, Ge Y, Zhao N, Hu X, Yuan Z
- Issue date: 2015 Mar
- Characterizing the microbiomes of Antarctic sponges: a functional metagenomic approach.
- Authors: Moreno-Pino M, Cristi A, Gillooly JF, Trefault N
- Issue date: 2020 Jan 20