Show simple item record

dc.contributor.authorGetsy, Paulina M
dc.contributor.authorSundararajan, Sripriya
dc.contributor.authorMay, Walter J
dc.contributor.authorvon Schill, Graham C
dc.contributor.authorMcLaughlin, Dylan K
dc.contributor.authorPalmer, Lisa A
dc.contributor.authorLewis, Stephen J
dc.date.accessioned2021-09-21T16:13:03Z
dc.date.available2021-09-21T16:13:03Z
dc.date.issued2021-09-15
dc.identifier.urihttp://hdl.handle.net/10713/16688
dc.description.abstractDecreases in arterial blood oxygen stimulate increases in minute ventilation via activation of peripheral and central respiratory structures. This study evaluates the role of endothelial nitric oxide synthase (eNOS) in the expression of the ventilatory responses during and following a hypoxic gas challenge (HXC, 10% O2, 90% N2) in freely moving male and female wild-type (WT) C57BL6 and eNOS knock-out (eNOS-/-) mice. Exposure to HXC caused an array of responses (of similar magnitude and duration) in both male and female WT mice such as, rapid increases in frequency of breathing, tidal volume, minute ventilation and peak inspiratory and expiratory flows, that were subject to pronounced roll-off. The responses to HXC in male eNOS-/- mice were similar to male WT mice. In contrast, several of the ventilatory responses in female eNOS-/- mice (e.g., frequency of breathing, and expiratory drive) were greater compared to female WT mice. Upon return to room-air, male and female WT mice showed similar excitatory ventilatory responses (i.e., short-term potentiation phase). These responses were markedly reduced in male eNOS-/- mice, whereas female eNOS-/- mice displayed robust post-HXC responses that were similar to those in female WT mice. Our data demonstrates that eNOS plays important roles in (1) ventilatory responses to HXC in female compared to male C57BL6 mice; and (2) expression of post-HXC responses in male, but not female C57BL6 mice. These data support existing evidence that sex, and the functional roles of specific proteins (e.g., eNOS) have profound influences on ventilatory processes, including the responses to HXC.en_US
dc.description.urihttps://doi.org/10.1038/s41598-021-97322-3en_US
dc.language.isoenen_US
dc.publisherSpringer Natureen_US
dc.relation.ispartofScientific Reportsen_US
dc.rights© 2021. The Author(s).en_US
dc.subjectarterial blood oxygenen_US
dc.subjecthypoxic gas challengeen_US
dc.subjectventilatory processesen_US
dc.subject.meshNitric Oxide Synthase Type IIIen_US
dc.subject.meshSex Factorsen_US
dc.subject.meshMiceen_US
dc.titleShort-term facilitation of breathing upon cessation of hypoxic challenge is impaired in male but not female endothelial NOS knock-out mice.en_US
dc.typeArticleen_US
dc.identifier.doi10.1038/s41598-021-97322-3
dc.identifier.pmid34526532
dc.source.journaltitleScientific reports
dc.source.volume11
dc.source.issue1
dc.source.beginpage18346
dc.source.endpage
dc.source.countryEngland


This item appears in the following Collection(s)

Show simple item record